【摘要】 为分析绿豆种质资源的遗传多样性，利用株高、主茎粗、主茎节数等10个表型性状及ISSR分子标记技术对48份绿豆种质资源进行变异系数、聚类分析和遗传多样性分析。结果表明，所有表型性状变异系数均大于8%,变异系数最大的表型性状为单株荚数(71.23%),其次为主茎茸毛密度(35.88%)和主茎茸毛颜色(35.20%)。表型性状聚类分析将供试材料分为五大类群。ISSR-PCR最终筛选出17条多态性有效引物，共检测274个位点，多态性位点百分率为83.57%,有效等位基因数为1.799,Nei’s基因多样性指数(期望杂合度)为0.434,Shannon’s信息指数为0.622,聚类分析将供试材料分为五大类群。供试材料表型性状分化程度高，具有丰富的遗传多样性。更多还原

【Abstract】 To analyze the genetic diversity of Vigna radiata germplasm resources, the coefficient of variation, cluster analysis and genetic diversity analysis of 48 Vigna radiata germplasm resources were conducted using 10 phenotypic traits such as plant height, main stem diameter and the number of main stem nodes, as well as ISSR molecular marker technology. The results showed that the coefficient of variation of all phenotypic traits was greater than 8%. The phenotypic trait with the highest coefficient of variation was the number of pods per plant(71.23%), followed by the density of main stem downy hairs(35.88%) and the color of main stem downy hairs(35.20%), respectively. Phenotypic trait cluster analysis classified the tested materials into five major groups. Finally, 17 polymorphic effective primers were screened out by ISSR-PCR. A total of 274 loci were detected. The percentage of polymorphic loci was 83.57%, the number of effective alleles was 1.799, the Nei’s genetic diversity index(expected heterozygosity) was 0.434, and the Shannon’s information index was 0.622. Cluster analysis classified the tested materials into five major groups. The phenotypic traits of the tested materials had a high degree of differentiation and rich genetic diversity更多还原