【摘要】 牡丹组织培养技术尚未应用于产业化的主要原因是试管苗生根后地上部发生休眠，导致移栽成活率低。研究发现，4℃低温处理牡丹生根试管苗60 d可以促进顶芽萌发，解除顶芽休眠。为了解析低温处理对试管苗顶芽萌发的相关机制，对低温处理不同天数的试管苗进行表型观察和转录组测序。结果表明，生根试管苗经低温处理后，抑制了ABI转录，ABA合成通路中一些关键基因PP2C、PYL、ARF等的表达受到抑制，阻碍了其生物合成，同时激活了GA生物通路中的一些关键基因GA2ox1、GAI的表达，促进了GA合成，导致试管苗中GA与ABA含量达到平衡，促进了顶芽萌发。更多还原

【Abstract】 The main reason why the tissue culture technology of Paeonia suffruticosa has not yet been applied to industrialization is that the above-ground parts of plantlet enter dormancy after rooting, resulting in a low survival rate after transplantation. Research has found that treating tree peony plantlets at a low temperature of 4 ℃ for 60 days could promote the germination of the apical bud and break the dormancy of the apical bud. In order to analyze the related mechanism of low-temperature treatment on the apical bud germination of plantlet, phenotypic observation and transcriptome sequencing were conducted on plantlet treated with low temperature for different days. The results indicated that after the rooting plantlet were treated with low temperature, the ABI transcription was inhibited, and the expressions of some key genes such as PP2C, PYL, and ARF in the ABA synthesis pathway were suppressed, hindering ABA biosynthesis. At the same time, the expressions of some key genes GA2ox1 and GAI in the GA biological pathway were activated, promoting GA synthesis led to the balance of GA and ABA contents in plantlet seedlings, promoting the germination of apical buds.更多还原