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管腔器械可视化系统联合ATP生物荧光检测在软式内镜清洗消毒效果评价中的应用

Application of Lumen Instrument Visualization System Combined with ATP Biofluorescence Detection in the Evaluation of Soft Endoscopic Cleaning and Disinfection Effect

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【作者】 范鹏超; 郭晓明; 刘文芝; 柳雯悦;

【Author】 FAN Pengchao;GUO Xiaoming;LIU Wenzhi;LIU Wenyue;Department of Disease Prevention and Hospital Infection Control, Second Affiliated Hospital of Dalian Medical University;Endoscopic Center, Second Affiliated Hospital of Dalian Medical University;Department of Otolaryngology, Second Affiliated Hospital of Dalian Medical University;

【通讯作者】 柳雯悦;

【机构】 大连医科大学附属第二医院疾病预防与医院感染控制部; 大连医科大学附属第二医院内镜中心; 大连医科大学附属第二医院耳鼻喉科;

【摘要】 目的 探讨管腔器械可视化系统联合ATP生物荧光检测技术在软式内镜干燥中的应用效果。方法 选取某三级甲等医院内镜中心2022年投入使用的48条胃肠镜作为研究对象,采取随机数字表法将其分为A、B、C三组,每组8条胃镜、8条肠镜,A、B、C三组干燥时间分别为1 min、5 min及10 min,除干燥时长外,其余清洗消毒步骤均按照规范要求同质化完成。利用管腔器械可视化系统联合ATP生物荧光检测对干燥后每条内镜进行检测。结果 检测的48条内镜中,有15条内镜管腔内无划痕,占全部检测内镜的31.25%;有4条内镜管腔有轻微划痕,占全部检测内镜的8.33%;有31条内镜管腔镜头前端有多处划痕,多条划痕贯彻整个镜子,占全部检测内镜的64.58%。检测的48条内镜中,仅有4条内镜管腔内存在锈迹,占全部检测内镜的8.33%,分别是A组肠镜2、肠镜6,B组肠镜12及C组肠镜17,锈迹大多分布在内镜的镜头前端或中下部。检测的48条内镜中,有3条内镜管腔内存在生物膜,占全部检测内镜的8.33%,分别是A组肠镜5、肠镜7及B组肠镜11。三组液滴计数及RLU值随着干燥时间的增加呈下降趋势,A组胃镜清洗消毒不合格率最高,为43.75%(7/16),A组与B组肠镜清洗消毒不合格率并列最高,为18.75%(3/16),随着干燥时间的增加不合格率随之降低,差异具有统计学意义(P<0.05)。结论 管腔器械可视化系统联合ATP生物荧光检测在软式内镜干燥效果评价中有着重要意义。临床清洗消毒时,应规范操作,避免腔隙内部损伤。同时,应增加内镜干燥时长,避免长期干燥失败导致的内镜相关感染事件。

【Abstract】 Objective To explore the application effect of lumen instrument visualization system combined with ATP biofluorescence detection technology in soft endoscopic drying effect.Methods The 48 gastroenteroscopy that was put into use by a certain tertiary A hospital endoscopy center in 2022 were selected as the research object.The random number table was adopted to divide it into three groups A,B,and C.Each group had 8 gastroscopy and 8 colonoscopy in total 16 endoscopy.The drying time of the three groups A,B,and C was 1 minute,5 minutes and 10 minutes respectively.Except for the drying time,the other cleaning and disinfection steps were homogeneously completed in accordance with the specification requirements.The lumen instrument visualization system and ATP biofluorescence detection were used to detect each endoscopy after drying.Results Among the 48 endoscopes tested,15 had no scratches in their lumens,accounting for 31.25% of all endoscopes tested;Four endoscopic lumens had slight scratches,accounting for 8.33% of all detected endoscopes;There are 31 scratches on the front end of the endoscope lumen lens,and multiple scratches run through the entire mirror,accounting for 64.58% of all detected endoscopes.Out of the 48 endoscopes tested,only 4 had rust inside their lumens,accounting for 8.33% of all endoscopes tested.They are Group A Colonoscopy 2,Colonoscopy 6,Group B Colonoscopy 12,and Group C Colonoscopy 17,with rust mostly distributed at the front or middle lower part of the endoscope lens.Among the 48 endoscopes tested,3 had biofilms in their lumens,accounting for 8.33% of all endoscopes tested.They are Group A colonoscope 5,colonoscope 7,and Group B colonoscope 11.The three groups of droplet counts and RLU values showed a decreasing trend with increasing drying time.Group A had the highest failure rate in gastroscopy cleaning and disinfection,at 43.75% (7/16),while Group A and Group B had the same highest failure rate in colonoscopy cleaning and disinfection,at 18.75% (3/16).The failure rate decreased with increasing drying time,and the difference was statistically significant (P<0.05).Conclusion The visualization system of luminal instruments combined with ATP bioluminescence detection is of great significance in evaluating the drying effect of soft endoscopes.During clinical cleaning and disinfection,standardized procedures should be followed to avoid internal damage to the cavity.At the same time,the duration of endoscopic drying should be increased to avoid endoscopic related infection events caused by long-term drying failures.

【基金】 国家卫健委医院管理研究所感研项目《基于风险评估的器官移植围术期全流程感控对策的研究》(项目编号:GY2023043)
  • 【分类号】R187
  • 【下载频次】23
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