【摘要】 角蛋白酶是一种重要的蛋白酶，它能降解羽毛等结构复杂的角蛋白，实现多肽、氨基酸等高值化产物的回收利用。目前，天然角蛋白酶的活性、抗逆性和生产效能难以满足工业化应用。蛋白质工程的发展为提高角蛋白酶的产量、改善功能特性提供了有效的途径。文章系统地综述了角蛋白酶的来源、生理特性及结构特点，总结了目前角蛋白酶活性检测的方法及存在的问题，重点探讨了角蛋白酶蛋白质工程在功能域改造及多功能域组合的工程方面的策略和研究进展，展望了未来角蛋白酶的主要研究方向。更多还原

【Abstract】 Keratinase is an important protease that can degrade complex keratin waste and enable the recycling of high-value products such as polypeptides and amino acids. Currently, the activity and biological yield of natural keratinase by hosting organisms are relatively low, severely hindering the application of keratinase. The development of protein engineering provides an effective means to increase the production of keratinase and improve its functional properties. This article systematically reviews the sources, physiological characteristics, and structural features of keratinase, summarizes the current methods of keratinase activity assay and the existing problems, and focuses on discussing the strategies and progress of keratinase protein engineering in the aspects of functional domain modification and multifunctional domain combination. Finally, we prospect the future application and directions of keratinase.更多还原