【摘要】 为了深入了解中国延边地区犬瘟热病毒（CDV）的流行及变异情况，本实验室对采集的10份疑似患CDV犬的口鼻拭子样品经胶体金试纸条检测后，对阳性样品进一步经RT-PCR检测，并扩增该病毒的各编码基因，采用DNAStar对各编码基因进行同源性及遗传进化分析，采用DataMonkey对该病毒株进行进化选择压力分析。结果显示：该样品经RT-PCR和胶体金检测均为CDV阳性，将该病毒株命名为CDV-YB；将CDV-YB H基因与世界各地CDV H基因序列对比分析并构建该基因的进化树，结果显示：CDV-YB株基因型为亚洲1型，其中与黑龙江ZYL株（KX488865）的亲缘关系最近，与美国Snyder Hill标准株（JN896987）的同源性为90.8%；采用DataMonkey预测CDVYB株的选择压力结果显示，该病毒株的7个关键蛋白均有不同方向的进化特征。上述结果表明CDV仍不断变异，亚型之间存在进化。本研究对CDV-YB株的各编码基因进行了遗传进化分析及选择压力分析，为CDV药物开发及疫苗制备提供了科学依据。更多还原

【Abstract】 In order to further understand the prevalence and variation of canine distemper virus(CDV) in Yanbian, China, our laboratory conducted RT-PCR and colloidal gold test strip assays on a swab sample collected from a suspected CDV-infected case,and amplified the whole genome of the virus. DNAStar was used for homology analysis and genetic evolution analysis of the whole genome and key proteins in the coding region. DataMonkey was used to analyze the evolutionary selection of this strain. The results showed that the sample was positive for CDV by RT-PCR, and the strain was named CDV-YB. Genetic evolution analysis: A comparison of the CDV-YB H gene with global CDV strains revealed that CDV-YB belongs to the Asia-1 genotype, with the closest genetic relationship to the Heilongjiang ZYL strain(KX488865) and a 90.8% homology to the Snyder Hill reference strain from the United States(JN896987). Data Monkey predicted the selection pressure acting on CDV-YB strain, showing that the 7 key proteins of CDV-YB strain exhibit distinct evolutionary characteristics. These results indicate that CDV continues to undergo genetic variation and evolutionary divergence among subtypes. This study provides genetic evolution and selection pressure analyses of the CDV-YB strain, offering, scientific basis for CDV drug development and vaccine preparation.更多还原