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西瓜萌芽期与幼苗期盐害参数的相关性分析
Correlation analysis of salt damage parameters during watermelon germination and seedling stages
【摘要】 为探究西瓜种子萌芽期与幼苗期耐盐性的相关性,以及萌芽期耐盐性是否能代表幼苗期耐盐性,本试验以16个西瓜品种为试验材料,测定各品种萌芽期和幼苗期的盐害相关指标(发芽势、发芽率、盐害指数、总鲜质量、地上部分鲜质量、叶绿素含量、丙二醛含量、脯氨酸含量)。采用主成分分析、隶属函数分析、相关性分析等方法对西瓜萌芽期盐害参数和幼苗期耐盐性进行综合评价。结果表明,NaCl处理后西瓜幼苗总鲜质量和地上鲜质量减少,丙二醛和脯氨酸含量上升。幼苗期综合隶属函数值范围为0.160~0.789,萌芽期综合隶属函数值范围为0.043~0.830。萌芽期各指标盐害参数聚类分析后将16个西瓜品种划分为5个耐盐等级。按照萌芽期分类的5个耐盐等级将萌芽期和幼苗期的综合隶属函数值和平均隶属函数值进行相关性分析后,发现萌芽期和幼苗期的皮尔逊相关系数分别达到0.727和0.826,萌芽期和幼苗期的耐盐等级之间达到了强相关水平,表明萌芽期的耐盐性在一定程度上能代表幼苗期耐盐性。研究结果为西瓜耐盐资源的早期筛选提供了理论依据。
【Abstract】 To investigate the correlation of salt tolerance between seed germination stage and seedling stage, and to determine whether salt tolerance during germination can serve as an indicator for salt tolerance in the seedling stage, 16 watermelon cultivars were used as experimental materials. Salt stress-related physiological and growth parameters—including germination potential, germination rate, salt injury index, total fresh masd, shoot fresh mass, chlorophyll content, malondialdehyde(MDA)content, and proline content—were measured at the germination and seedling stages. Principal component analysis, membership function analysis, and correlation analysis were employed to comprehensively evaluate salt tolerance traits at both developmental stages. The results indicated that NaCl treatment significantly reduced total fresh mass and shoot fresh mass of seedlings, while increasing MDA and proline content. The comprehensive membership function value at the seedling stage ranged from 0.160 to 0.789. Based on key indicators such as salt injury index, fresh mass,shoot fresh mass, chlorophyll content, MDA content, and proline content, the 16 cultivars were classified into five salt tolerance grades using cluster analysis. Similarly, at the germination stage, the comprehensive membership function values ranged from 0.043 to 0.830, and the cultivars were grouped into five salt tolerance classes. Correlation analysis between the average and comprehensive membership function values at the two stages—according to the five-grade classification system established at the germination stage—revealed Pearson correlation coefficients of 0.727 and 0.826, respectively, indicating a strong positive correlation between salt tolerance at the germination and seedling stages. These findings suggest that salt tolerance at the germination stage may be a reliable predictor of salt tolerance performance at the seedling stage in watermelon. The research results provide a theoretical basis for the early screening of watermelon salt-tolerant resource.
【Key words】 Watermelon; Salt stress; Membership function; Principal component analysis; Correlation;
- 【文献出处】 中国瓜菜 ,China Cucurbits and Vegetables , 编辑部邮箱 ,2025年12期
- 【分类号】S651
- 【下载频次】8