【摘要】 为快速检测新乡小包23越冬制种基地受冻害地块杂交种纯度，确保流入市场的种子质量达标，维护消费者权益，同时提高杂交种筛选效率，从12对大白菜InDel引物中筛选能够区分大白菜杂交种新乡小包23及其父母本的特异性引物并进行种子纯度鉴定。结果表明，有3对引物表现出共显性，其中1对引物能够将新乡小包23与另外3个相似品种有效区分开。将该引物在108个杂交后代中进行验证，纯度鉴定结果为93.5%，与田间鉴定结果基本一致。综上，研究筛选的InDel标记BrID90105可用于新乡小包23种子纯度的快速鉴定，促进种业良性发展。更多还原

【Abstract】 To rapidly detect the purity of hybrid seeds from frozen damaged plots in the overwintering seed production base of Xinxiangxiaobao 23, ensure that the quality of seeds entering the market meets standards, protect consumers rights, and improve the efficiency of hybrid seed screening, specific primers were selected from 12 pairs of InDel primers for Chinese cabbage to distinguish Xinxiangxiaobao 23 and its parents, and seed identification was carried out. The results showed that three pairs of primers exhibited co-dominance, among which one pair could effectively distinguish Xinxiangxiaobao 23 from the other three similar varieties. This primer was verified in 108 hybrid progenies, and the purity identification result was 93.5%, which was basically consistent with the field identification result. In conclusion, the InDel marker BrID90105 selected in this study can be used for rapid identification of seed purity in Xinxiangxiaobao 23,promoting the healthy development of the seed industry.更多还原