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玉米麸皮膳食纤维复合酶法改性工艺优化
Optimization of Process of Corn Bran Dietary Fiber Modified by Compound Enzymatic Method
【摘要】 作为玉米淀粉加工的副产物,玉米麸皮(CB)含有丰富的总纤维,是膳食纤维的良好来源。目前研究表明,CB作为一种生物质资源,与麦麸、米糠等其他副产物相比,其膳食纤维的含量更高。以玉米麸皮可溶性膳食纤维(SDF)得率为主要指标,结合吸附特性,通过Plackett-Burman设计试验和单因素试验确定最佳的复合酶法改性工艺:纤维素酶与木聚糖酶同步添加,添加比例为1∶1,酶用量为4 mg/mL,料液比为1∶20,在初始pH 4.5、温度45℃的条件下水解12 h。与单酶法改性相比,复合酶法改性处理后SDF得率可达8.01%,持水力、持油力、膨胀力分别为4.09 g/g、2.42 g/g、2.39 mL/g,吸附特性得到改善。该研究结果可为玉米麸皮膳食纤维的产业化开发奠定理论基础。
【Abstract】 As a by-product of corn starch processing, corn bran(CB) is rich in total fiber, which is a good source of dietary fiber.Current studies show that CB is a biomass resource, and the content of dietary fiber in it is higher than other by-products such as wheat bran and rice bran. With the yield of soluble dietary fiber(SDF) in corn bran as the main index, combined with the adsorption properties, the best compound enzymatic modification process is determined by Plackett-Burman design test and single factor test: cellulase and xylanase are added at the same time, the ratio is 1∶1, the enzyme dosage is 4 mg/mL, the solid-liquid ratio is 1∶20, and it is hydrolyzed at initial pH 4.5 and enzymatic hydrolysis temperature 45 ℃ for 12 h. Compared with the single enzymatic modification, the yield of SDF after compound enzymatic modification can reach 8.01%, the water holding capacity, oil holding capacity and swelling capacity are 4.09 g/g, 2.42 g/g and 2.39 mL/g respectively, and the adsorption properties are improved. The results can lay a theoretical foundation for the industrial development of corn bran dietary fiber.
【Key words】 corn bran; dietary fiber; enzymatic modification; adsorption properties;
- 【文献出处】 中国调味品 ,China Condiment , 编辑部邮箱 ,2025年03期
- 【分类号】TS210.9
- 【下载频次】128