【摘要】 目的 探讨新疆哈萨克族T2DM患者外周血中长链非编码RNA（LncRNA） GUSBP3和LncRNA SAM4的表达水平及诊断价值。方法 选取2019年1～9月于石河子大学第一附属医院门诊或住院治疗的哈萨克族T2DM患者30例为T2DM组,另选同期体检健康者30名为正常对照（NC）组,比较两组一般资料、生化指标及差异表达的LncRNA水平。Spearman相关分析目的基因与各指标的相关性。Logstic回归分析T2DM的影响因素。受试者工作特征（ROC）曲线评估目的基因的诊断价值。结果 两组差异表达的LncRNA为LncRNA GUSBP3、LncRNA SAM4。T2DM组DBP、FPG、HbA₁c、果糖胺、FIns、胰岛β细胞功能指数、血肌酐（Scr）、LncRNA GUSBP3、LncRNA SAM4表达高于NC组（P<0.05）。Spearman相关性分析显示,LncRNA GUSBP3表达与Scr、血尿酸（SUA）呈正相关（r=0.328、0.322,P<0.05）,与FIns呈负相关（r=-0.295,P<0.05）。LncRNA SAM4表达与SUA呈正相关（r=0.275,P<0.05）,与LDL-C呈负相关（r=-0.212,P<0.05）。Logistic回归分析显示,DBP（OR 1.175,95%CI 1.011～1.367）、FIns （OR 0.852,95%CI 0.739～0.982）、LncRNA GUSBP3 （OR0.378,95%CI 0.149～0.958）为哈萨克人口T2DM的影响因素。LncRNA GUSBP3诊断T2DM的ROC曲线下面积为0.789。结论 哈萨克族T2DM患者LncRNA GUSBP3、LncRNA SAM4表达水平高于健康人,其表达水平与部分生化指标相关,DBP、FIns、LncRNA GUSBP3是哈萨克族人口患T2DM的影响因素,LncRNA GUSBP3可能成为诊断新疆哈萨克族T2DM的生物标志物。更多还原

【Abstract】 Objective To investigate the expression levels and diagnostic value of long noncoding RNA(LncRNA)GUSBP3 and LncRNA SAM4 in peripheral blood of Kazakh type 2 diabetes mellitus(T2DM) patients in Xinjiang. Methods From January to September 2019,Kazakh T2DM patients in The First Affiliated Hospital of Shihezi University were recruited through outpatient or inpatient department(T2DM group,n=30). And 30 healthy people were selected as normal control(NC) group during the same period. The general data,biochemical indicators and differentially expressed LncRNA levels were compared between the two groups. Spearman correlation analysis was used to analyze the correlation between the target genes and each index. Logistic regression was used to analyze the influencing factors for T2DM. Receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of target genes. Results The differentially expressed LncRNA were LncRNA GUSBP3 and LncRNA SAM4 in the two groups. DBP,FPG,Hb A1c,fructosamine,FIns,islet β cell function index,serum creatinine(Scr),LncRNA GUSBP3 and LncRNA SAM4 were higher in the T2DM group than in the NC group(P<0. 05). Spearman correlation analysis showed that the expression of LncRNA GUSBP3 was positively correlated with Scr and serum uric acid(SUA)(r=0. 328,0. 322,P<0. 05),and negatively correlated with FIns(r=-0. 295,P <0. 05). The expression of LncRNA SAM4 was positively correlated with SUA(r=0. 275,P<0. 05) and negatively correlated with LDL - C(r=-0. 212,P<0. 05). Logistic regression analysis showed that DBP(OR 1. 175,95%CI 1. 011～1. 367),FIns(OR 0. 852,95%CI 0. 739～0. 982), LncRNA GUSBP3(OR 0. 378,95%CI 0. 149～0. 958) was influencing factors for T2DM in Kazakh population. The area under the ROC curve of LncRNA GUSBP3 was 0. 789 for T2DM diagnosis. Conclusions The expression levels of LncRNA GUSBP3 and LncRNA SAM4 were higher in Kazakh patients with T2DM than in healthy people,and their expression levels are related to some biochemical indicators. DBP,FIns and LncRNA GUSBP3 are the influencing factors for T2DM in Kazakh population. LncRNA GUSBP3 may be a biomarker for the diagnosis of T2DM in Kazakh in Xinjiang.更多还原