【摘要】 为了改善红曲色素的稳定性和溶解性，以明胶和阿拉伯胶为壁材，采用复合凝聚法制备红曲色素纳米胶囊。通过测定可溶性复合物的浊度、粒径和PDI确定复合凝聚反应中壁材比例和pH值。结果显示，明胶∶阿拉伯胶质量比为1∶1,pH值为4.7时，最有利于壁材的复合凝聚反应。在此基础上，探究壁材浓度、芯材浓度、固化剂浓度对纳米胶囊的影响，结果表明，当壁材质量浓度7.5 mg/mL、芯材质量浓度7.5 mg/mL、红曲色素质量浓度15 mg/mL、固化剂质量浓度3 mg/mL、乳化剂体积分数0.5%时可获得较均一稳定的纳米胶囊。此条件下纳米胶囊包埋率为95.46%，粒径均值为99.81 nm,PDI为0.174,zeta电位为-15.56 mV，溶解度达95%。对该纳米胶囊的光稳定性、贮藏稳定性及体外释放性能表征分析，发现红曲色素的贮藏稳定性和光稳定性得到明显提高，纳米胶囊中红曲红色素和橙色素符合一级降解动力学模型，黄色素遵循零级降解动力学模型，拟合数据显示色素纳米胶囊的半衰期明显延长，光稳定性提高。此外，红曲色素纳米胶囊具备一定的靶向肠道释放功能，可实现在模拟胃液中较少释放和在模拟肠液中大量释放，这表明纳米包埋体系能提高红曲色素的生物利用度。更多还原

【Abstract】 In order to improve the stability and solubility of Monascus pigment, nanocapses were prepared by composite coagulation method with gelatin and gum acacia as wall materials. The wall material ratio and pH conditions of the complex coacervation reaction were determined by measuring the turbidity curve, particle size and PDI of the soluble complex stage. The results showed that the ratio of gelatin and gum arabic was 1∶1 and the pH was 4.7, which was most beneficial to the composite cohesion reaction of wall materials. On this basis, the effects of wall material concentration, core material concentration, and curing agent concentration on nanocapsules were explored. The results showed that the most uniform and stable nano-capsules could be obtained when the wall material concentration was 7.5 mg/mL, the core material concentration was 7.5 mg/mL, the Monascus pigment content was 15 mg/mL, the curing agent addition was 3 mg/mL,and the volume fraction of the emulsifier addition was 0.5%. Under these conditions, the encapsulation rate of nanocapsules was 95.46%, the mean particle size was 99.81 nm, the PDI was 0.174, the zeta potential was-15.56 mV, and the solubility reached 95%. The photostability, storage stability, and in vitro release activity of the nanocapsules were characterized. The results showed that the storage stability and photostability of Monascus pigment were significantly improved. The degradation kinetics of red pigment and orange pigment in nanocapsules followed a first-order degradation model, while the degradation kinetics of yellow pigment followed a zero-order degradation model. The fitting data showed that the half-life of the pigment nanocapsules was significantly prolonged and the light stability was improved. In addition, the moascus pigment nanocapsules possessed certain intestinal targeted release function, with less release in simulated gastric fluid and greater release in simulated intestinal fluid, indicating that the nano-embedding system could improve the bioavailability of Monascus pigments.更多还原