【摘要】 目的 对比不同质量浓度的番泻叶灌胃联合慢性束缚应激法构建的腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome, IBS-D)小鼠模型差异性，以确定适宜的番泻叶灌胃质量浓度。方法 根据相关研究中构建IBS-D小鼠模型使用的番泻叶灌胃质量浓度，制定质量浓度梯度，并开展实验。雌性C57BL/6小鼠随机分成空白组(N组)、低剂量组(0.25 g/mL番泻叶，L组)、中剂量组(0.50 g/mL番泻叶，M组)和高剂量组(1.0 g/mL番泻叶，H组),每组10只。连续造模14 d后，观察并记录各组小鼠排便情况、腹泻指数、内脏敏感性及结肠组织形态学变化，比较不同番泻叶质量浓度构建的模型差异。结果 与N组小鼠(42.90±11.90)%相比，L组(80.30±5.77)%、M组(80.50±3.44)%、H组(81.90±2.68)%小鼠的6 h粪便含水率显著增加(P<0.01);与N组小鼠(0.00±0.00)相比，L组(0.57±0.16)、M组(0.62±0.23)、H组(0.60±0.23)小鼠的腹泻指数也均显著增加(P<0.01)。与N组小鼠(0.65(0.60, 0.65))相比，M组(0.32(0.24, 0.39))和H组(0.34(0.27, 0.47))小鼠的内脏痛阈值显著降低，内脏敏感性增高(P<0.01)。此外，M组小鼠首粒蓝便时间(98.15(93.41, 100.44))min较N组小鼠(186.81(109.28, 192.05))min显著缩短(P<0.01)且M组小鼠的6 h粪便总粒数(22.4±3.73)也较N组小鼠(17.90±4.48)相比明显增加(P<0.05)。结论 与0.25和1.0 g/mL相比，0.50 g/mL的番泻叶灌胃联合慢性束缚应激能更好的构建IBS-D小鼠模型。更多还原

【Abstract】 Objective This study sought to establish a diarrhea-predominant irritable bowel syndrome(IBS-D) mouse model by gavage different mass concentrations sennae folium combined with chronic restraint stress, and to determine the appropriate mass concentration of sennae folium to establish IBS-D mouse model. Methods The mass concentration of sennae folium used for the IBS-D mouse model followed suggested amounts in the literature and on that basis, the mass concentration gradient was established prior to conducting the experiment. Female C57BL/6 mice were divided into a normal group(Group N), a low-dose group(Group L; 0.25 g/mL sennae solution), a medium-dose group(Group M; 0.50 g/mL sennae solution), and a high-dose group(Group H; 1.0 g/mL sennae solution), with 10 mice per group. After 14 days, the defecation, diarrhea index, visceral sensitivity, and morphological changes in the colonic tissue in each group were observed and recorded to compare the differences among models established with varying mass concentrations of sennae folium. Results Compared with Group N(42.90 ± 11.90)%, Group L(80.30 ± 5.77)%, Group M(80.50 ± 3.44)%, and Group H(81.90 ± 2.68)% had significantly higher 6 h fecal water content(P < 0.01). Compared with Group N(0.00 ± 0.00), the diarrhea index of mice in Group L(0.57 ± 0.16), Group M(0.62 ± 0.23), and Group H(0.60, 0.23) also increased significantly(P < 0.01). Compared with Group N(0.65(0.60, 0.65)), Group M(0.32(0.24, 0.39)) and Group H(0.34(0.27, 0.47)) had significantly lower visceral pain threshold and higher visceral sensitivity(P < 0.01). Additionally, the first blue stool time in Group M(98.15(93.41, 100.44) min) was significantly shorter than that in Group N(186.81(109.28, 192.05) min)(P < 0.01), and the total number of stools in Group M(22.4 ± 3.73) was significantly higher than that in Group N(17.90 ± 4.48)(P < 0.05). Conclusions Compared with 0.25 and 1.0 g/mL, 0.50 g/mL sennae folium gavage, combined with chronic restraint stress, can better simulate the clinical symptoms of IBS-D.更多还原