【摘要】 目的 了解江西省上栗县婴幼儿肠道病毒A组71型(Enterovirus A71,EV-A71)、柯萨奇病毒A组6型(Coxsackievirus A6,CV-A6)、柯萨奇病毒A组16型(Coxsackievirus A16,CV-A16)和柯萨奇病毒A组10型(Coxsackievirus A10,CV-A10)血清流行率。方法 在上栗县招募10～16月龄和22～33月龄健康儿童并采集血清标本，采用固定病毒法检测EV-A71、CV-A16、CV-A6和CV-A10中和抗体，分析抗体阳性率和几何平均滴度(Geometric average titer,GMT)。结果 在230例调查儿童中，EV-A71、CV-A6、CV-A16和CV-A10中和抗体阳性率分别为65.22%、61.30%、13.48%、3.04%,GMT(1∶)分别为32.15、220.90、6.54、4.25。0剂次、1剂次、2剂次EV-A71疫苗接种史者EV-A71中和抗体阳性率分别为3.85%、80.00%、99.24%(χ~2=198.80,P<0.001),GMT(1∶)分别为4.18、16.86、118.32(F=194.87,P<0.001);其中2剂次EV-A71疫苗接种后≤90 d、91～365 d、366～720 d阳性率分别为100%、97.06%、100%(Fisher确切概率法，P=0.258),GMT(1∶)分别为229.99、94.17、91.16(F=5.15,P=0.007)。结论 调查地区儿童2剂次EV-A71疫苗接种显著提高了EV-A71抗体水平，但存在较高水平的既往CV-A6自然感染。更多还原

【Abstract】 Objective To assess seroprevalences of enterovirus A71(EV-A71)and Coxsackievirus A6,A16,and A10(CV-A6,CV-A16,and CV-A10)in infants and young children of Shangli county in Jiangxi province.Methods We recruited children 10-16 and 22-23 months of age in Shangli and obtained serum samples from the children. We tested for neutralizing antibodies against EV-A71,CV-A6,CVA16,and CV-A10 using the fixed virus method to determine positivity rates and geometric mean titers(GMT).Results Among 230 children,neutralizing antibody positivity rates against EV-A71,CV-A6,CV-A16,and CV-A10 were 65. 22%,61. 30%,13. 48%,and 3. 04%,respectively,with GMTs(1∶)of 32. 15,220. 90,6. 54,and 4. 25. EV-A71 neutralizing antibody positivity rates among children with0-,1-,and 2-dose EV-A71 vaccination histories were 3. 85%,80. 00%,and 99. 24%(χ~2= 198. 80,P<0. 001),with GMTs of 4. 18,16. 86,and 118. 32(F = 194. 87,P < 0. 001); positivity rates ≤90,91-365,and 366-720 days after 2 doses were 100%,97. 06%,and 100%(Fisher’ s exact test,P =0. 258),with GMTs(1 ∶)of 229. 99,94. 17,and 91. 16(F = 5. 15,P = 0. 007). Conclusions Two doses of EV-A71 vaccine significantly increased EV-A71 antibody levels among children in the surveyed area. There was a high level of natural infection with CV-A6.更多还原