【摘要】 目的 通过生物信息学的方法分析着丝粒蛋白A在宫颈癌(CC)患者中的表达及临床意义。方法 在TCGA癌症基因组图谱、GEO基因表达数据库及GEPIA、UALCAN数据库中提取CC相关的RNA测序数据，评估着丝粒蛋白A的表达及其在CC患者中的临床意义；使用R语言提取TCGA数据库中的CC患者的临床病理参数，并利用χ~2检验、单因素及多因素COX回归分析等检验方法对数据进行分析；使用在线工具对GEO的两个CC数据集分析，并对CENP-A进行GO功能分析。收集CC患者及健康对照患者外周血标本，qRT-PCR方法检测CENP-A mRNA表达水平的差异。结果 与正常宫颈组织相比，CC组织中的着丝粒蛋白A表达显著上调(P<0.05),CENP-A的表达量与患者的年龄、CC分期、肿瘤浸润深度、远处转移、AJCC分期及病理类型无明显关系，与淋巴结转移呈正相关(P<0.05);多因素COX回归分析显示，淋巴结转移是影响CC患者预后的独立危险因素(P<0.05);对着丝粒蛋白A进行GO功能富集分析，发现其主要富集在“有丝分裂”“染色体区域”及“蛋白质结合”等。CC患者外周血有核细胞中CENP-A的含量明显高于对照组(P<0.05)。结论 着丝粒蛋白A在CC组织中表达上调且与CC患者的淋巴结转移情况相关，可能为CC患者的分子诊断和治疗提供潜在的研发靶点。更多还原

【Abstract】 Objective Bioinformatics was used to investigate centromere protein A expression and clinical relevance in cervical cancer(CC) patients.Methods Using the CC RNA sequencing data from the TCGA dataset, GEO gene expression database, GEPIA and UALCAN database to evaluate the expression of centromere A and its clinical significance in CC.Using R language to analyze the clinicopathologic parameters of CC in the TCGA dataset with χ~2 test and univariate and multivariate COX regression analysis.Using online tools to analyze two CC data sets from GEO,and analyze the GO function of CENP-A.Peripheral blood samples from CC patients and healthy controls were collected, and the qRT-PCR method was used to detect the difference in CENP-A mRNA expression levels.Results CENP-A expression was significantly up-regulated in CC compared to normal cervical tissues(P<0.05).The expression level of CENP-A had no significant relationship with the age of patients, CC stage, depth of tumor invasion, lymph node metastasis, AJCC stage and pathological type, but was positively correlated with distant metastasis(P<0.05).Multivariate COX regression analysis showed that was independent risk factor for the CC.GO enrichment analysis showed that centromere protein A was mainly enriched in "mitosis", "chromosome region" and "protein binding".The content of CENP-A in peripheral blood nucleated cells in CC patients was significantly higher than that in the control group(P<0.05).Conclusion Centromere protein A is up-regulated in CC tissues, which may be related to distant metastasis in CC patients, and may promote the development of targeted precision oncology.更多还原