【摘要】 目的 研究腺样体肥大患儿鼻咽微生物菌群的多样性和丰度变化。方法 收集2021年6月—2022年6月就诊于湖南省人民医院耳鼻咽喉科的30例腺样体肥大患儿的相关临床资料，其中男18例，女12例，平均年龄6.6岁。按照A/N比值测量，其中腺样体轻度、中度、重度肥大各10例。采集腺样体表面分泌物，采用16S核糖体RNA(16S rRNA)方法分析鼻咽微生物群，测序后进行生物信息学分析(BMkCloud,www. biocloud.net)。结果 腺样体肥大患儿鼻咽物种多样性及丰度发生变化。在重度腺样体肥大患儿中，嗜血杆菌属及莫拉菌属所占比例较高(各占比例40.19%及24.93%)，链球菌属所占比例较低(占比为14.66%)。结论 腺样体肥大患儿鼻咽物种细菌丰度发生变化，嗜血杆菌属，莫拉菌属的丰度增加和链球菌属丰度减少可能与腺样体肥大的发生发展相关，为临床进一步了解腺样体肥大的微生物机制提供帮助。更多还原

【Abstract】 Objective To study the changes in diversity and abundance of nasopharyngeal microbiota in children with adenoid hypertrophy.Methods Clinical data of 30 children with adenoid hypertrophy who visited the Department of Otolaryngology,Hunan People ’s Hospital from June 2021 to June 2022 were collected,including 18 males and 12 females,with an average age of 6.6 years.Adenoidal surface secretions were collected for analysis of the nasopharyngeal microbiota using the 16S ribosomal RNA( 16S rRNA) method.After sequencing,bioinformatics analysis was conducted( BMkCloud,www.biocloud.net).Results The species diversity and abundance of nasopharyngeal microbiota were changed in children with adenoid hypertrophy.In the severe adenoid hypertrophy group,the proportions of Haemophilus and Moraxella were significantly higher( 40.19% and 24.93%),while that of Streptococcus was significantly lower( 14.66%).Conclusions The bacterial abundance of nasopharyngeal species changes in children with adenoidal hypertrophy.The abundance of Haemophilus and Moraxella increases,while that of Streptococcus decreases,which may be related to the occurrence and development of adenoid hypertrophy.This is helpful to understand the microbial mechanism of adenoid hypertrophy in clinic.更多还原