【摘要】 目的 探究血液学与神经表达因子1(hematological and neurological expressed 1,HN1)在卵巢癌（ovarian cancer,OC）中的表达及其潜在的作用机制。方法 利用基因表达谱交互式分析平台（gene expression profiling interactive analysis,GEPIA）、Kaplan-Meier生存曲线和阿拉巴马大学伯明翰分校开发的综合性癌症数据分析平台（the University of Alabama at Birmingham Cancer Data Analysis Portal,UALCAN）等在线数据库，分析HN1基因在泛癌中的表达水平及其在OC中的表达差异及预后价值。基于癌症基因组图谱数据库筛选与HN1共同表达的基因，并进行基因本体（Gene Ontology,GO）和京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes,KEGG）富集分析。进一步通过R软件分析HN1表达水平与OC组织中免疫细胞浸润程度之间的关联性。结果 GEPIA数据库分析显示HN1在包括OC在内的20种肿瘤组织的表达显著高于正常组织（P<0.05）。UALCAN数据库结果表明HN1在低分化OC患者中的表达水平明显升高（P<0.05）。生存分析显示HN1高表达患者的无进展生存期、总生存期及疾病进展后生存期均显著低于HN1低表达患者（P<0.05）。GO和KEGG富集分析结果表明，HN1可能主要参与细胞周期调控生物过程。免疫相关分析发现HN1表达水平与CD4~+Th2细胞和树突状细胞的浸润程度呈显著正相关（P<0.05）。结论 HN1在OC中呈高表达，并可能通过调控细胞周期、促进上皮–间充质转化及影响免疫细胞浸润参与肿瘤的发生发展过程。HN1有望成为OC诊断及靶向治疗的潜在分子标志物。更多还原

【Abstract】 Objective To investigate the expression pattern and potential functional mechanisms of hematological and neurological expressed 1(HN1) in ovarian cancer(OC). Methods The gene expression profiling interactive analysis(GEPIA), Kaplan-Meier plotter, and the University of Alabama at Birmingham Cancer Data Analysis Portal(UALCAN) online databases were utilized to analyze the expression profile of HN1 across pan-cancer datasets, as well as its differential expression and prognostic significance in OC. Co-expressed genes of HN1 were identified based on the cancer genome atlas database, followed by functional enrichment analyses using Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways. Furthermore, the association between HN1 expression and immune cell infiltration in OC tissues was explored by using R software. Results Analysis of the GEPIA database revealed that HN1 expression was significantly elevated in 20 types of tumor tissues, including OC, compared to normal tissues(P<0.05). UALCAN data indicated that HN1 expression was markedly higher in poorly differentiated OC patients(P<0.05). Kaplan-Meier survival analysis showed that patients with high HN1 expression had significantly shorter progression-free survival, overall survival, and post-progression survival compared to those with low HN1 expression(P<0.05). GO and KEGG enrichment analyses suggested that HN1 is mainly involved in the regulation of the cell cycle. Immunological analysis demonstrated that HN1 expression levels were positively correlated with the infiltration of CD4+ Th2 cells and dendritic cells in OC tissues(P<0.05). Conclusion HN1 is highly expressed in OC and may play a role in tumor progression by regulating cell cycle, promoting epithelial-mesenchymal transition, and modulating immune cell infiltration. HN1 has the potential to serve as a novel biomarker for the diagnosis and targeted therapy of OC.更多还原