【摘要】 目的 研究刺茶美登木Maytenus variabilis的倍半萜生物碱类化学成分及其细胞毒活性。方法 运用正相硅胶柱色谱、制备薄层色谱及制备型HPLC等方法进行化合物分离纯化，并用1D NMR、2D NMR、HRMS等现代波谱技术进行化合物的结构鉴定。采用MTT法研究化合物的抗肿瘤活性。结果 从刺茶美登木中分离鉴定2个倍半萜生物碱类化合物，分别鉴定为：（1R,2S,4S,5S,6R,7R,9R,10R）-1,2-dinicotinoyloxy-4-hydroxy-6,15-diacetoxy-9-benzoyloxy-dihydro-β-agarofuran（1）和（1R,2S,4S,5S,6R,7R,9R,10R）-1,6,15-triacetoxy-2,9-dinicotinoyloxy-4-hydroxy-dihydro-β-agarofuran（2）。结论 化合物1和2为新的倍半萜生物碱类化合物，分别命名为美登木宁碱A及美登木宁碱B。细胞毒活性研究显示，化合物1和2对人胃癌HGC-27细胞有一定的体外增殖抑制活性，其半数抑制浓度(median inhibition concentration,IC₅₀)分别为69.44、65.74μmol/L。更多还原

【Abstract】 Objective To study the sesquiterpene alkaloids from Maytenus variabilis and their cytotoxic activities. Methods The compounds were isolated and purified by column chromatography over normal phase silica gel, preparative thin-layer chromatography and preparative HPLC. Their structures were elucidated by means of various spectroscopic techniques including one-dimensional nuclear magnetic resonance（1D NMR）, two-dimensional nuclear magnetic resonance（2D NMR） and high resolution mass spectrometry（HRMS）. The anti-tumor activity of the compound was investigated by MTT assay. Results Two sesquiterpene alkaloids were isolated from M. variabilis, their structures were elucidated as（1R,2S,4S,5S,6R,7R,9R,10R）-1,2-dinicotinoyloxy-4-hydroxy-6,15-diacetoxy-9-benzoyloxy-dihydro-β-agarofuran（1） and（1R,2S,4S,5S,6R,7R,9R,10R）-1,6,15-triacetoxy-2,9-dinicotinoyloxy-4-hydroxy-dihydro-β-agarofuran（2）, respectively. Conclusion The cytotoxic activity study revealed that compounds 1 and 2 exhibited certain in vitro proliferation inhibitory activity against human gastric cancer HGC-27 cell with IC₅₀ values of 69.44, 65.74 μmol/L.更多还原