【摘要】 目的：探讨褪黑素能否通过抑制铁死亡减轻急性心肌梗死。方法：H9C2细胞在低糖无血清AnaeroPack系统中培养10 h，建立急性心肌梗死细胞模型。然后用褪黑素和铁死亡诱导剂erastin处理细胞。检测细胞活性、活性氧、脂质过氧化、线粒体膜电位和铁死亡相关蛋白表达。通过皮下注射异丙肾上腺素诱导急性心肌梗死大鼠模型，观察褪黑素对大鼠心肌梗死大小、心肌损伤、病理改变、氧化应激、铁离子及铁死亡相关蛋白表达的影响。结果：褪黑素可降低缺氧诱导的心肌细胞氧化应激、脂质过氧化和铁死亡相关蛋白的表达，但这些作用可被铁死亡诱导剂erastin抑制。此外，在体内实验中，我们还发现褪黑素可改善心肌梗死大小、心脏损伤、病理改变、氧化应激，减轻铁离子积累和铁死亡。结论：褪黑素对心肌梗死的心脏保护作用与抑制心肌铁死亡有关。更多还原

【Abstract】 AIM:To investigate whether melatonin can ameliorate acute myocardial infarction (AMI) by inhibiting ferroptosis.METHODS:H9C2 cells were cultured in AnaeroPack system with low sugar and serum-free medium for 10 h to construct a cell model of AMI.Then cells were treated with melatonin and ferroptosis inducer erastin.The cell activity,reactive oxygen species(ROS),lipid peroxidation,mitochondrial membrane potential(MMP),and ferroptosis related protein expression were detected.A rat model of AMI induced by isoprenaline(ISO) injection was established to evaluate the effects of melatonin,in which the myocardial infarction size,cardiac injury,pathological changes,oxidative stress,iron ion and ferroptosis related protein expression were examined.RESULTS:Melatonin decreased the oxidative stress,lipid peroxidation and expression of ferroptosis protein in cardiomyocytes induced by hypoxia,but these effects could be impeded by the ferroptosis inducer erastin.Furthermore,in vivo experiments,we also found that melatonin improved the myocardial infarction size,cardiac injury,pathological changes,oxidative stress,and alleviated iron ion accumulation and ferroptosis.CONCLUSION:The cardioprotective effects of melatonin in AMI are associated with the inhibition of ferroptosis.更多还原