【摘要】 采用改良麦克马斯特饱和盐水浮漂法、聚合酶链式反应（PCR）及测序技术进行病原体的分子鉴定和系统发育分析。镜检结果显示，总感染率为7.96%，霍城县的感染率（27.27%）显著高于其他地区（p<0.01），吉木萨尔县的感染率最低（1.31%）。在检测的粪便虫卵中，绦虫感染率（4.47%）高于线虫（3.98%）。PCR进一步鉴定出细粒棘球绦虫（0.49%）、泡状带绦虫（0.49%）和犬弓首蛔虫（3.48%）。值得注意的是，泡状带绦虫与犬弓首蛔虫的混合感染率为2.98%，细粒棘球绦虫和犬弓首蛔虫混合感染未检出。系统发育分析表明，本研究获得的分离株与Gen Bank数据库中的参考序列有90%以上的同源性。研究揭示了北疆犬类寄生虫的多样性，需加大防控力度降低细粒棘球绦虫等寄生虫的传播风险。更多还原

【Abstract】 Using the modified McMaster saturated saline flotation method, polymerase chain reaction(PCR), and sequencing technology, molecular identification and phylogenetic analysis of the pathogens are conducted. Microscopic examination reveals an overall infection rate of 7.96%, with the infection rate in Huocheng County(27.27%) being significantly higher than in other areas(p<0.01), while Jimsar County has the lowest infection rate(1.31%). Among the detected fecal eggs, the infection rate of cestodes(4.47%) is higher than that of nematodes(3.98%). PCR further identified Echinococcus granulosus(0.49%), Taenia hydatigena(0.49%), and Toxocara canis(3.48%). Notably, the co-infection rate of Taenia hydatigena and Toxocara canis is 2.98%, while no co-infection of Echinococcus granulosus and Toxocara canis is detected. Phylogenetic analysis shows that the isolate obtained in this study shows more than 90% homology with the reference sequences in the GenBank database. The study reveals the diversity of canine parasites in northern Xinjiang and highlights the need for enhanced prevention and control measures to reduce the transmission risks of parasites such as Echinococcus granulosus.更多还原