【摘要】 目的 探讨双参通脉颗粒（GSG）是否通过抑制细胞外活化蛋白激酶1/2-核因子-κB（ERK1/2-NF-κB）信号通路改善急性心肌梗死（AMI）模型大鼠的心肌损伤。方法 将60只SD大鼠分为假手术组（A组，等量生理盐水），模型组（B组，等量生理盐水），阿托伐他汀组（C组，8 mg/kg）,GSG低、中、高剂量组（D₁组、D₂组、D₃组，5,10,15 g/kg），各10只。除A组外，其余各组大鼠通过左冠状动脉前降支结扎建造AMI模型，造模成功后24 h灌胃相应药物。检测各组大鼠的心功能；采用酶联免疫吸附试验（ELISA）法检测血清炎性因子，按试剂盒操作检测生化指标；分别采用2,3,5-氯化三苯基四氮唑（TTC）染色法、苏木精-伊红（HE）染色法、原位末端标记（TUNEL）法检测心肌梗死、病理损伤及细胞凋亡情况；采用免疫印迹（Western blot）法检测细胞凋亡与通路相关蛋白表达情况。结果 与B组比较，D₁组、D₂组、D₃组大鼠左心室收缩压（LVSP）、平均动脉压（MAP）、左心室内压上升最大速率(+dp/dt_max)、B细胞淋巴瘤-2（Bcl-2）表达水平均显著升高（P<0.05），左心室内压下降最大速率(-dp/dt_max)、左心室舒张末压（LVEDP）、肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白1（MCP-1）、白细胞介素（IL）-6、IL-1β、肌钙蛋白I（cTnI）、肌酸激酶同工酶（CK-MB）、心肌梗死程度、心肌细胞凋亡率、胱天蛋白酶-3（caspase-3）、B淋巴细胞瘤2相关X蛋白（Bax）、p-ERK1、p-ERK2、NF-κB p65表达水平均显著降低（P<0.05）。结论 GSG可能通过抑制ERK1/2-NF-κB信号通路改善AMI模型大鼠的心肌损伤。更多还原

【Abstract】 Objective To investigate whether Shuangshen Tongmai Granules can improve myocardial injury in acute myocardial infarction （AMI） model rats by inhibiting the extracellular signal-regulated kinases 1/2-nuclear factor-κB （ERK1/2-NF-κB） signaling pathway.Methods A total of 60 SD rats were divided into the sham operation group （group A,equal volume of phgsiological saline）,model group （group B,equal volume of phgsiological saline）,atorvastatin group （group C,8 mg/kg）,and low-,medium-,and high-dose Shuangshen Tongmai Granules groups （groups D₁,D₂,D₃,5,10,15 g/kg）,with 10 rats in each group.Except for rats in group A,rats in the other groups were used to establish AMI models by ligating the left anterior descending coronary artery.At 24 h after successful modeling,corresponding drugs were administered by gavage.The cardiac function of rats in each group was detected.The serum inflammatory factors were detected by the enzyme-linked immunosorbent assay （ELISA）,and biochemical indicators were detected according to the test kits.Myocardial infarction,pathological damage,and cell apoptosis were detected by the TTC staining method,hematoxylin eosin （HE） staining method,and TdT-mediated dUTP nick end labeling （TUNEL） method,respectively.The expression of apoptosis-related protiens and pathway-related proteins in cells was detected by the Western blot.Results Compared with those in group B,the left ventricular systolic pressure （LVSP）,mean arterial pressure （MAP）,maximal left ventricular pressure rising rate (+dp/dt_max),and expression levels of B-cell lymphoma 2 （Bcl-2） significantly increased in groups D₁,D₂,and D₃,while the maximal left ventricular pressure decreasing rate(-dp/dt_max),left ventricular end-diastolic pressure （LVEDP）,tumor necrosis factor-α （TNF-α）,monocyte chemoattractant protein-1 （MCP-1）,interleukin （IL）-6,IL-1β,cardiac troponin I （cTnI）,creatine kinase-MB （CK-MB）,degree of myocardial infarction,myocardial cell apoptosis rate,the expression levels of caspase-3,BCL2-associated X protein （Bax）,p-ERK1,p-ERK2,and NF-κB p65 significantly decreased in groups D₁,D₂,and D₃（P<0.05）.Conclusion Shuangshen Tongmai Granules may improve myocardial injury in rats with myocardial infarction by inhibiting ERK1/2-NF-κB signaling pathway.更多还原