【摘要】 目的 探究艾灸对自然衰老小鼠肝脏脂质代谢的影响。方法 选取18月龄C57BL/6J自然衰老雌性小鼠24只按照随机数字表法分为老年组、艾灸组、阳性组，每组8只；8只6月龄青年雌性小鼠作为空白组。空白组和老年组不进行特殊干预；艾灸组每日麦粒灸5壮，每周6次，共8周；阳性组采用饮食限制8周。采用HE染色观察肝脏形态，油红O染色观察脂滴蓄积水平；比较各组血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）水平；比较各组肝组织白细胞介素（IL）-1α、IL-1β含量；RT-qPCR法和Western blot法检测m TOR、AMPK、ULK1 mRNA和蛋白表达。结果 与空白组比较，老年组肝细胞出现炎性浸润、脂肪变性、脂滴相对面积增大等病理学改变；与老年组比较，艾灸组出现不同程度的减轻。与空白组比较，老年组肝脏指数、脂滴相对面积升高（P<0.05）；与老年组比较，艾灸组肝脏指数、脂滴相对面积降低（P<0.01）。与空白组比较，老年组血清TG、TC、LDL-C水平升高，血清HDL-C水平降低（P<0.01）；与老年组比较，艾灸组血清TG、TC、LDL-C水平降低，血清HDL-C水平升高（P<0.05或P<0.01）。与空白组比较，老年组肝组织IL-1α、IL-1β水平升高（P<0.01）；与老年组比较，艾灸组肝组织IL-1α、IL-1β水平降低（P<0.01）。与空白组比较，老年组肝组织m TOR mRNA和蛋白表达升高，AMPK、ULK1 mRNA和蛋白表达降低（P<0.05或P<0.01）；与老年组比较，艾灸组肝组织m TOR mRNA和蛋白表达降低，AMPK、ULK1 mRNA和蛋白表达升高（P<0.05或P<0.01）。结论 艾灸改善肝组织脂质代谢与衰老标志物水平，可能与m TOR/AMPK/ULK1信号通路相关。更多还原

【Abstract】 Objective To investigate the effect of moxibustion on lipid metabolism in the liver of natural aging mice.Methods Twenty-four 18-month-old natural aging female C57 BL/6J mice were selected and divided into elderly group,moxibustion group, and positive group according to the random number table method, with eight mice in each group; and eight 6-month-old young female mice were used as blank group. No special intervention was given to the blank group and elderly group; the moxibustion group received five moxibustion sessions with wheat grains every day, six times a week, for a total of eight weeks; and the positive group was subject to dietary restriction for eight weeks. The liver morphology was observed by HE staining, and the lipid droplet accumulation level was observed by oil red O staining; the levels of serum triglycerides(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), and high-density lipoprotein cholesterol(HDL-C) of each group were compared; the contents of interleukin(IL)-1α and IL-1β in liver tissues of each group were compared; and the expressions of m TOR, AMPK, and ULK1 mRNA and protein were detected by RT-q PCR and Western blot. Results Compared with the blank group, pathological changes such as inflammatory infiltration, steatosis and increased relative area of lipid droplets occurred in hepatocytes of the elderly group; compared with the elderly group, the moxibustion group showed varying degrees of alleviation. Compared with the blank group, the liver index and the relative area of lipid droplets in the elderly group increased(P <0.05); compared with the elderly group, the liver index and relative area of lipid droplets in the moxibustion group decreased(P <0.01). Compared with the blank group, the levels of serum TG, TC, and LDL-C in the elderly group increased, while the level of serum HDL-C decreased(P<0.01); compared with the elderly group, the levels of serum TG, TC, and LDL-C in the moxibustion group decreased, while the level of serum HDL-C increased(P<0.05 or P<0.01). Compared with the blank group, the levels of IL-1α and IL-1βin liver tissues of the elderly group increased(P<0.01); compared with the elderly group, the levels of IL-1α and IL-1β in the liver tissues of the moxibustion group decreased(P<0.01). Compared with the blank group, the expressions of m TOR mRNA and protein in liver tissues of the elderly group increased, while the expressions of AMPK and ULK1 mRNA and protein decreased(P<0.05 or P<0.01); compared with the elderly group, the expressions of m TOR mRNA and protein in liver tissues of moxibustion group decreased, while the expressions of AMPK and ULK1 mRNA and protein increased(P<0.05 or P<0.01). Conclusion Moxibustion can improve liver lipid metabolism and aging marker levels, which may be related to the regulation of the AMPK/m TOR/ULK1 signaling pathway.更多还原