【摘要】 目的 探讨牛磺酸上调基因1(taurine-upregulated gene 1,TUG1)对人脐静脉内皮细胞(human umbilical vein endothelial cell, HUVEC)在氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation, OGD/R)损伤后凋亡、增殖、迁移等生物学功能的影响。方法 OGD/R处理HUVEC建立OGD/R损伤的细胞模型，分别转染沉默TUG1的小干扰RNA(small interfering RNA-TUG1, si-TUG1, si-TUG1)及其空白对照(si-NC),将细胞分为对照组，OGD/R组、OGD/R+si-NC组和OGD/R+si-TUG1组，PCR检测细胞TUG1的表达水平，流式细胞术检测各组细胞凋亡，CCK-8实验检测细胞增殖，划痕实验检测细胞迁移，免疫荧光检测细胞中CD31表达，Western blot法检测细胞中核增殖抗原(proliferating cell nuclear antigen, PCNA)蛋白的表达。结果 PCR结果显示，OGD/R组细胞中TUG1的表达水平显著高于对照组(P<0.05);转染si-TUG1可显著降低HUVEC中TUG1的表达水平(P<0.05);si-TUG1组HUVEC的凋亡率显著低于si-NC组(P<0.05);si-TUG1组HUVEC的增殖、迁移、CD31免疫荧光强度以及PCNA蛋白表达水平均高于si-NC组(P<0.05)。结论 降低TUG1的表达则可显著减少OGD/R条件下HUVEC的凋亡，增加其增殖、迁移、成管能力和细胞活性。更多还原

【Abstract】 Objective To investigate the effects of taurine-upregulated gene 1(TUG1) on the biological functions of human umbilical vein endothelial cells(HUVEC) such as apoptosis, proliferation, and migration after oxygen-glucose deprivation/reoxygenation(OGD/R) injury. Methods HUVEC were treated with OGD/R to establish a cell model injured by OGD/R. Small interfering RNA silencing TUG1(si-TUG1) and its negative control(si-NC) were transfected, and the cells were divided into the control group, OGD/R group, OGD/R+si-NC group, and OGD/R+si-TUG1group. PCR was used to detect the expression level of TUG1 in HUVEC; flow cytometry was used to detect cell apoptosis; CCK-8 assay was used to detect cell proliferation; wound-healing assay was used to detect cell migration; immunofluorescence was used to detect the expression of CD31 in HUVEC; and Western blot was used to detect the expression of nuclear proliferation antigen(PCNA) protein. Results The PCR results showed that the expression level of TUG1 in OGD/R group cells was significantly higher than that in the control group(P<0.05); si-TUG1 transfection can significantly reduce the expression level of TUG1 in HUVEC(P<0.05); the apoptosis rate of HUVEC in the si-TUG1group was significantly lower than that in the si-NC group(P<0.05); The cell proliferation, cell migration, immunofluorescence intensity of CD31, and the expression levels of PCNA protein in the si-TUG1group were higher than those in the si-NC group(P<0.05). Conclusion Inhibiting TUG1 can significantly reduce the apoptosis of HUVEC under OGD/R conditions, and increase the biological functions of HUVEC, such as proliferation, migration, and tubular ability.更多还原