【摘要】 目的 通过超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF/MS）联用技术定性分析中药复方麻杏石甘汤中的主要化学成分。方法 采用UPLC BEH C18柱(2.1 mm×100 mm,2.5μm)，柱温40℃，以0.4 ml/min流速的0.1%甲酸水-0.1%甲酸乙腈流动相梯度洗脱，分析时间为15 min；质谱条件为电喷雾离子源(ESI)，正、负离子全扫描模式采集质谱数据。结果 通过UPLC-QTOF/MS分析和对照品验证，共鉴别出麻杏石甘汤组方中59种化学成分。结论 建立了一种超高效液相色谱-四级杆飞行时间质谱(UPLC-QTOF/MS)联用方法鉴别中药麻杏石甘汤的化学成分，该方法简便高效，灵敏准确，为麻杏石甘汤药效物质基础与作用机制的深入研究提供依据，有助于中药治疗新冠肺炎的组方配伍优化和质量控制。更多还原

【Abstract】 Objective To analyze chemical constituents of compound Maxing Shigan decoction by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS). Methods The separation was performed on a UPLC BEH C18 column(2.1 mm×100 mm, 2.5 μm),with a gradient elution applying 0.1% aqueous formic acid solution and 0.1% formic acid acetonitrile as a mobile phase. The column temperature was 40 °C. The flow rate was 0.4 ml/min and the analysis time was 15 min. Mass spectrometry(MS) data were collected in both positive and negative ESI ion modes. Results Through UPLC-QTOF/MS analysis and reference validation, a total of 59 chemical components in Maxing Shigan decoction were identified.Conclusion An ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS)method was established to identify the chemical components of Maxing Shigan decoction. This method is simple, efficient, sensitive and accurate, and provides a basis for the elucidation of the pharmacodynamic material basis and mechanism of Maxing Shigan decoction. It can provide data reference for the optimization of the compatibility of traditional Chinese medicine in the treatment of COVID-19.更多还原