【摘要】 目的:研究黑色素瘤相关抗原D4(MAGED4)与蛋白质赖氨酸脱酰基酶Sirtuin 5(SIRT5)在人胶质瘤组织与细胞中的表达情况，并探讨二者表达相关性。方法:通过基因表达谱交互式分析平台2(GEPIA2)分析681例胶质瘤组织与207例正常脑组织间MAGED4与SIRT5 mRNA表达情况以及二者mRNA表达相关性。使用人类蛋白质图谱数据库(HPA)研究了10例胶质瘤组织切片中MAGED4蛋白表达情况与35例胶质瘤组织切片中SIRT5蛋白表达情况，并进行胶质母细胞瘤(GBM)患者的生存分析。通过免疫组织化学(IHC)分别检测60例胶质瘤组织切片中MAGED4与SIRT5蛋白表达情况，并分析二者蛋白表达与患者临床病理参数相关性。利用实时荧光定量PCR(RT-qPCR)与蛋白质免疫印迹(WB)检测下调胶质瘤细胞MAGED4表达对SIRT5 mRNA与蛋白表达的影响。结果:GEPIA2分析显示，MAGED4 mRNA在低级别胶质瘤(LGG)与GBM组织的表达均高于正常脑组织;而SIRT5 mRNA在LGG和GBM组织的表达与正常脑组织无显著差异。HPA分析结果显示，胶质瘤组织切片中MAGED4阳性表达率为90%; SIRT5阳性表达率为74.29%。IHC结果显示，MAGED4高表达27例、中等表达23例、低表达7例、阴性表达3例; SIRT5高表达34例、中等表达10例、低表达9例、阴性表达7例。相关性分析结果显示，二者表达具有显著正相关性(r=0.369,P<0.05)。患者临床病理参数的相关性分析结果表明，MAGED4与肿瘤大小、SIRT5表达水平呈显著相关(P<0.05)，但与其他各临床资料无明显相关性(P>0.05); SIRT5与除MAGED4外的其他各临床资料无明显相关性(P>0.05)。结论:MAGED4与SIRT5在胶质瘤中高表达，且呈正相关;胶质瘤细胞中MAGED4可影响SIRT5的表达。更多还原

【Abstract】 Objective: To investigate the expression of melanoma-associated antigen D4(MAGED4) and protein lysine deacylase Sirtuin 5(SIRT5) in human glioma tissues and cells,and to explore the correlation between their expressions.Methods: The Gene Expression Profiling Interactive Analysis 2(GEPIA2) was used to analyze the mRNA expression of MAGED4 and SIRT5 in 681 glioma tissues and 207 normal brain tissues,as well as the correlation between their mRNA expressions. The Human Protein Atlas(HPA) database was used to analyze the expression of MAGED4 protein in 10 glioma tissue sections and the expression of SIRT5 protein in 35 glioma tissue sections,and performed survival analysis for glioblastoma multiforme(GBM) patients. Immunohistochemistry(IHC) was used to detect the expression of MAGED4 and SIRT5 proteins in60 human glioma tissue sections,and the correlation between their protein expressions and the clinical pathological parameters of the patients was analyzed. Real-time fluorescence quantitative PCR(RT-qPCR) and protein Western blot(WB) were used to detect the effect of down-regulating the expression of MAGED4 in glioma cells on the expression of SIRT5 mRNA and protein. Results: GEPIA2 analysis showed that the expression of MAGED4 mRNA in low-grade glioma(LGG) and glioblastoma(GBM) tissues was higher than that in normal brain tissues; there was no significant difference in the expression of SIRT5 mRNA in LGG and GBM tissues compared with normal brain tissues. HPA analysis showed that the positive expression rate of MAGED4 in glioma tissue sections was 90%; the positive expression rate of SIRT5 was 74.29%. IHC results showed that MAGED4 was highly expressed in 27 cases,moderately expressed in 23 cases,lowly expressed in 7 cases,and negatively expressed in 3 cases; SIRT5 was highly expressed in 34 cases,moderately expressed in 10 cases,lowly expressed in 9 cases,and negatively expressed in 7 cases. Correlation analysis showed that the expression of the two was significantly positively correlated(r=0.369,P<0.05). The results of the correlation analysis of clinicopathological parameters in patients showed that MAGED4 was significantly correlated with tumor size and SIRT5 expression levels(P<0.05),but had no significant correlation with other clinical data(P>0.05). SIRT5 showed no significant correlation with other clinical data except for MAGED4.Conclusion: MAGED4 and SIRT5 are highly expressed in glioma and are positively correlated. MAGED4 can affect the expression of SIRT5 in glioma cell.更多还原