【摘要】 目的 利用形态学与分子生物学的方法对2023年内蒙古自治区包头市采集到的伊蚊属蚊虫进行种类鉴定。方法 2023年包头市成蚊监测时采集到伊蚊属蚊虫，通过形态学方法检索蚊虫特征，并同时提取蚊虫基因组DNA,用线粒体细胞色素c氧化酶亚基Ⅰ(cytochrome c oxidase subunit I,CO I)基因和内在转录间隔区2(internal transcribed spacer 2,ITS2)的基因片段进行PCR扩增和测序，测序结果在GenBank数据库中进行比对，采用P-distance参数法计算遗传距离。为验证方法的准确性，对中国疾病预防控制中心传染病预防控制所媒介生物控制室饲养的埃及伊蚊进行PCR扩增及序列分析。结果 包头市伊蚊属蚊虫样本形态学鉴定为仁川伊蚊，其CO I和ITS2基因碱基序列在GenBank数据库中未比对出相似性>98.0%的同源序列，而获得的CO I和ITS2基因碱基序列分别相互比对相似性在98.5%以上。中国疾病预防控制中心实验室饲养的埃及伊蚊的CO I基因序列与GenBank数据库中的埃及伊蚊基因序列相似性为98.5%～98.9%,ITS2基因序列相似性为94.7%～99.7%。结论 包头市采集的伊蚊属蚊虫形态学鉴定为仁川伊蚊，GenBank数据库中无仁川伊蚊的相关序列信息。本文在世界上首次将仁川伊蚊的CO I和ITS2基因序列上传至GenBank数据库，为全世界蚊虫分子生物学分类提供依据。更多还原

【Abstract】 Objective To identify the species of mosquito and insect of the genus Aedes mosqutio and inset collected in Baotou City of Inner Mongolia Autonomous Region in 2023 using morphological and molecular biological methods.Methods Mosquito and insect of the genus Aedes mosqutio and inset were collected for adult mosquito surveillance in Baotou City in 2023.Mosquito and insect characteristics were retrieved by morphological methods and the genome DNA of mosquito and insect was simultaneously extracted.Gene fragments of mitochondrial cytochrome c oxidase subunit I(CO I) gene and internal transcribed spacer 2(ITS2) were used for PCR amplification and sequencing.The sequencing results were compared in GenBank database and genetic distances were calculated using the P-distance parameter method.In order to verify the accuracy of the method, PCR amplification and sequence analysis were performed on Aedes aegypti reared in the Vector Biology Control Room of the Institute of Infectious Disease Prevention and Control of Chinese Center for Disease Control and Prevention.Results The morphological identification of samples of the genus Aedes mosqutio and inset in Baotou City were Aedes chemulpoensis.The CO I gene and ITS2 gene base sequences were not compared in the GenBank database for homologous sequences with >98.0% similarity, but the obtained CO I and ITS2 gene base sequences were similar to each other with more than 98.5% similarity, respectively.The similarity of CO I gene sequences of Aedes aegypti reared in the laboratory of Chinese Center for Disease Control and Prevention with those in the GenBank database ranged from 98.5% to 98.9%,and the similarity of ITS2 gene sequences ranged from 94.7% to 99.7%.Conclusion The genus Aedes mosquito and insect collected in Baotou City are morphologically identified as Aedes chemulpoensis.There is no related sequence information of Aedes chemulpoensis in the GenBank database.The CO I and ITS2 gene sequences of Aedes chemulpoensis are uploaded to GenBank database for the first time in the world, providing a basis for the molecular biology classification of mosquito and insect around the world.更多还原