【摘要】 目的 基于鼠疫相关病原和抗体检测青海省同德县喜马拉雅旱獭样本，分析2022年青海省同德县鼠疫流行态势，为今后鼠疫免疫流行病学研究和鼠疫防控策略制定提供理论依据。方法 2022年6—9月自青海省同德县采集327份喜马拉雅旱獭的肝脾脏器和血液样本，利用细菌学方法、反向间接血凝试验(reverse indirect hemagglutination test, RIHA)和间接血凝试验(indirect hemagglutination test, IHA)3种方法调查该地区鼠疫流行情况；采集193份喜马拉雅旱獭盲肠样本分离鼠疫菌噬菌体；基于双层琼脂平板法和点滴法检测327份喜马拉雅旱獭血清中鼠疫菌噬菌体免疫抗体。结果 基于鼠疫细菌学和血清学方法2022年青海省同德县327份喜马拉雅旱肝脾脏器样本和血清样本均未检出鼠疫的阳性材料；从4份喜马拉雅旱獭盲肠内容物样本中分离4株鼠疫菌噬菌体，间接说明该地区喜马拉雅旱獭不久前可能被鼠疫菌感染过。结论 同德县鼠疫疫源地喜马拉雅旱獭盲肠样本中检出鼠疫菌噬菌体，提示该地区鼠疫监测工作仍不容忽视。更多还原

【Abstract】 Objective To analyze the epidemic situation of plague in Tongde County, Qinghai Province in 2022 based on plague-related pathogens and antibodies detection in Marmota himalayana samples, this study provides a theoretical basis for future immunoepidemiology research on plague and the formulation of prevention and control strategies.Methods From June to September 2022,327 liver, spleen, and blood samples were collected from Marmota himalayana in Tongde County, Qinghai Province.Plague prevalence in this region was assessed using the bacteriological method, reverse indirect hemagglutination test(RIHA),and indirect hemagglutination test(IHA).Additionally, 193 cecal samples of Marmota himalayana were collected for Yersinia pestis Phage isolate.The presence of Yersinia pestis Phage-neutralizing antibodies in the 327 samples of Marmota himalayana serum was detected using the double-layer agar plate method and the drop method.Results Among 327 Marmota himalayana spleen and serum samples in Tongde County, Qinghai Province in 2022,no positive results were detected by bacteriological and serological methods for plague.However, four Yersinia pestis Phages were isolated from cecal content samples of Marmota himalayana,suggesting possible recent Yersinia pestis infection in this local Marmota himalayana.Conclusion Yersinia pestis Phage is detected in the cecal samples of Marmota himalayana Tongde County plague epidernic focus, indicating that the surveillance of plague in this area should not be ignored.更多还原