【摘要】 目的 建立尿碘的高基质进样-电感耦合等离子体质谱(high matrix introduction-inductively coupled plasma mass spectrometry, HMI-ICP-MS)分析方法，并应用于临床尿碘检测，以了解2019年广州市儿童尿碘的摄入情况，为制定碘缺乏病防制措施提供科学依据。方法 通过调节雾化气、稀释气和反应气流量获得最佳HMI条件，通过对检出限、精密度、准确度进行测定，评估HMI-ICP-MS对尿碘的测定方法，对广州市儿童尿碘进行非参数秩和检验统计分析。结果 当雾化气流量为0.4ml/min,稀释气流量为0.7ml/min,碰撞气流量为4.4ml/min时，HMI可以获得最佳工作条件。尿碘浓度在1.0～40.0μg/L时，该方法线性良好，相关系数为0.999 9～1.000 0;以3倍[信噪比(signal-to-noise ratio, S/N)=3]确定的检出限为0.088μg/L,尿碘加标回收率为71.3%～101.3%,相对标准偏差为1.2%～2.4%。2019年广州市577名8～10岁男童的尿碘中位数为203.4μg/L,566名女童的尿碘中位数为197.0μg/L,不同性别儿童尿碘水平差异无统计学意义(Z=20.703,P=0.931)。结论 高基质进样-电感耦合等离子体质谱法方法准确度高，精密度好，满足方法要求，该方法具有更高的耐受大量高盐样品检测的能力。性别本身对尿碘水平影响不大。更多还原

【Abstract】 Objective To establish a method for the analysis of urinary iodine by high matrix introduction-inductively coupled plasma mass spectrometry(HMI-ICP-MS) and apply to the clinical detection of urinary iodine, to understand the urinary iodine intake of children in Guangzhou City in 2019,and to provide a scientific basis for the development of iodine deficiency disease prevention and control measures.Methods The optimal HMI condition was obtained by adjusting the flow rate of atomization gas, dilution gas and reaction gas.The detection limit, precision and accuracy were measured to evaluate the method of determination of urinary iodine by HMI-ICP-MS.The urinary iodine of children in Guangzhou was statistically analyzed by the non-parametric rank sum test.Results When atomizing gas flow rate was 0.4ml/min, diluting gas flow rate was 0.7ml/min, collision gas flow rate was 4.4ml/min, HMI could obtain the best working conditions.In the range of 1.0-40.0μg/L of urineiodine concentration, the linearity of the method was good, and the correlation coefficient was 0.999 9-1.000 0.The detection limit was 0.088μg/L determined by a 3-fold signal-to-noise ratio(S/N=3).The spiked recovery rate of urinary iodine was 71.3%-101.3%,with a relative standard deviation of 1.2%-2.4%.In 2019,the median urinary iodine concentration of 577 boys aged 8-10 years in Guangzhou City was 203.4μg/L,and the median urinary iodine concentration of 566 girls was 197.0μg/L.There was no significant difference in urinary iodine levels between children of different genders(Z=20.703,P=0.931).Conclusion The HIM-ICP-MS method has high accuracy and good precision meeting the methodological requirements.This method has a higher ability to tolerate the detection of a large number of high-salt samples.Gender itself has little impact on urinary iodine levels.更多还原