【摘要】 以染料孟加拉红为研究对象，优化黄曲霉A5P1脱色孟加拉红的脱色条件。基于细胞固定化技术，考察了在不同pH条件下的固定化脱色效果，构建了聚氨酯泡沫（PUF）强化黄曲霉A5P1（菌株A5P1）脱色孟加拉红体系，考察固定化前后不同染料初始质量浓度、盐质量浓度和金属离子质量浓度对A5P1脱色孟加拉红的影响。结果表明：在pH为3的条件下，以蔗糖和酵母提取物为碳氮源时，黄曲霉A5P1对孟加拉红的脱色率最高；在pH为4的条件下，PUF固定化强化脱色体系的脱色效果最优。在染料质量浓度为300和500 mg/L，盐质量浓度为5～80 g/L、Cd2+质量浓度为5和10 mg/L、Pb2+质量浓度为100 mg/L的条件下，PUF固定化强化脱色系统的脱色效果优于游离系统。PUF固定化强化脱色体系在较高染料质量浓度和含NaCl的高盐条件仍能保持较好的脱色效果，对Pb2+和Cd2+金属离子的耐受性显著提高。黄曲霉A5P1对孟加拉红有较好的降解效果，PUF强化菌株A5P1脱色体系的良好性能使其在孟加拉红生物修复中具有潜在的应用价值。更多还原

【Abstract】 The present study focuses on the optimization of decolorization conditions for dye Rose Bengal using the fungus Aspergillus flavus A5P1(strain A5P1). By employing immobilized cell technology, the decolorization efficiency was investigated under different pH conditions. Polyurethane foam(PUF) was used to enhance the decolorization process, the effects of initial dye concentration, salt concentration, and metal ion concentration before and after immobilization were examined. The results showed that at pH 3 with sucrose and yeast extract as carbon and nitrogen sources, strain A5P1 exhibited the highest decolorization ratio for Rose Bengal. Under pH 4, PUF-immobilized system demonstrated optimal enhancement in decolorization efficiency compared to free system. When dye concentrations were 300 and 500 mg/L, salt concentrations ranged from 5 g/L to 80 g/L, Cd2+ concentrations were 5 and 10 mg/L respectively, Pb2+ concentration was set at 100mg/L; PUF-immobilized enhanced system outperformed free system in terms of decolorization efficiency.PUF-immobilized enhanced system maintained good performance even under high dye concentrations and high salt conditions containing NaCl, and exhibited significantly improved tolerance towards Pb2+ and Cd2+. The excellent degradation capability of strain A5P1 towards Rose Bengal suggests its potential application value in bioremediation processes involving this particular dye.更多还原