【摘要】 TCP基因家族在植物叶形建成方面具有重要调节功能。以蕈树Altingia chinensis全基因组数据为依据，利用生物信息学方法，对AcTCP基因家族进行鉴定，并对其基因结构、蛋白理化性质、染色体和蛋白亚细胞定位、共线性、启动子顺式作用元件和转录组表达模式进行分析。结果表明，从蕈树基因组中鉴定到21个Ac TCPs。所有Ac TCPs均含有TCP superfamily结构域，只有AcTCP3单独具有nitrilase superfamily结构域。蕈树TCPs分为PCF(AcTCP1/2/3/4/7/8/9/11/12/17/20/21)、CIN(AcTCP5/6/13/15/16/19)和CYC/TB1(AcTCP10/14/18) 3类。Ac TCPs蛋白包含197～554个氨基酸，亚细胞定位除Ac TCP3位于细胞膜，其余均位于细胞核，均为不稳定亲水蛋白。Ac TCPs基因的启动子含有光、激素、胁迫及生长发育相关元件。转录组表达分析表明，Ac TCPs基因存在组织特异性，其中AcTCP6/7/16在叶-Ⅰ(3月取样)中特异高效表达，但同一亚家族大部分成员的表达模式相似。从21个AcTCPs成员中挖掘出AcTCP16、AcTCP19和AcTCP20三个与蕈树叶形发育相关的关键潜在基因，推测蕈树TCPs在光、植物生长发育、激素和胁迫响应中发挥重要作用。更多还原

【Abstract】 The TCP gene family plays a crucial regulatory role in leaf-shape development in plants. This paper aims to identify and analyze the AcTCPs gene family in Altingia chinensis, exploring their tissue expression pattern to provide a reference for understanding the expression and function of TCPs in this species. Utilizing the whole genome data of A. chinensis and bioinformatics methods, we identified the AcTCPs gene family and analyzed theirs gene structure, protein physicochemical properties, chromosome localization, subcellular localization, collinearity, cis-acting elements of promoters, and transcription expression patterns in different tissues. The results showed that 21 AcTCPs were obtained of the A. chinensis genome. All AcTCPs contain the TCP superfamily domain, with only Ac TCP3 uniquely possessing the nitrilase superfamily domain. The AcTCPs can be classified into three categories: PCF(Ac TCP1/2/3/4/7/8/9/11/12/17/20/21), CIN(Ac TCP5/6/13/15/16/19) and CYC/TB1(AcTCP10/14/18). The AcTCP proteins range from 197 to 554 amino acids in length. Subcellular localization analysis revealed that Ac TCP3 is located at the cell membrane, while the others are localized in the nucleus, all of which are unstable hydrophilic proteins. The promoters of Ac TCPs genes contain light-responsive elements, hormone-responsive elements, stress-responsive elements and growth and development-related elements. Transcriptomic expression analysis revealed that tissue specificity for AcTCPs, with Ac TCP6, Ac TCP7, and AcTCP16 showing significantly high expression in leaf-I(March), although most members of the same subfamily exhibited similar expression patterns. This study identified a total of 21 AcTCP members and highlighted three key potential genes–Ac TCP16, Ac TCP19 and Ac TCP20 that are associated with leaf development in A. chinenesis. It is inferred that A. chinenesis TCPs play important roles in light response, plant growth and development, hormone signaling, and stress response. The findings provide a scientific basis for further studies on the genomic characteristics of the TCP gene family.更多还原