【摘要】 为了从白星花金龟幼虫虫粪中分离筛选具有除氨效果的微生物，为有机废弃物转化利用提供微生物资源。采用富集驯化、初筛、复筛相结合的方式从白星花金龟幼虫虫粪中筛选出2株除氨菌种N1、N2，结合形态、生理生化、16S r DNA分子鉴定菌株类别，对菌株的生理性状和生长曲线进行测定；并通过硼酸吸收凯氏定氮法测定单菌株和复配菌株在25 d内对NH₃释放的影响。结果表明：N1和N2均表现出了明显的除氨能力，复配菌株的除氨效果高于单菌株。经鉴定N1为小麦苍白杆菌，N2为苍白杆菌属。N1和N2生长曲线各具特点。两菌株在30～37℃pH=5～9时生长情况最好。从白星花金龟幼虫虫粪中分离的2株菌株能有效降低NH₃释放量，2株菌株复配的除氨效果高于单菌株，可作为有机废弃物在发酵腐熟期间有效的除氨微生物。更多还原

【Abstract】 To isolate and screen microorganisms with ammonia removal effect from the feces of the larvae of Protaetia brevitarsis,and to provide microbial resources for the conversion and utilization of organic waste,2strains of ammonia-removing bacteria N1 and N2 were screened from the feces of the larvae of P.brevitarsis by means of enrichment and domestication,preliminary screening and re-screening.The strains were identified by morphological,physiological and biochemical,and 16SrDNA molecular identification.The physiological characteristics and growth curve of the strain were determined.The effects of single strain and compound strain on NH₃ release within 25 days were determined by boric acid absorption Kjeldahl method.The findings indicated that both N1 and N2 showed obvious ammonia removal ability,and the ammonia removal effect of the compound strain was higher than that of the single strain.N1 was identified as Ochrobactrum tritici,and N2 was identified as Ochrobactrum sp.The growth curves of N1 and N2 had their own characteristics.The 2 strains grew best a30℃–37℃pH=5–9.The 2 strains isolated from the larvae of P.brevitarsis can effectively reduce the release of NH₃,and the ammonia removal effect of the 2 strains is higher than that of the single strain,which can be used as an effective ammonia removal microorganism during the fermentation of organic waste.更多还原