【摘要】 探究仙客来的花粉活力、柱头可授性的变化及不同种杂交后花粉管生长情况，为仙客来杂交育种提供依据。以6个仙客来主栽品种为材料，对花粉萌发培养基、不同开花天数仙客来各品种的花粉活力、柱头可授性进行试验及观察，并通过荧光显微镜观察3个正反杂交组合花粉管萌发及生长情况。结果显示：不同品种仙客来最佳花粉萌发培养基有明显差异；不同开花天数花粉活力不同，‘蝶衣重瓣’在开花第2～3 d花粉生活力最强，其余各品种花粉在开花第2～5 d有较高花粉活力，第5 d后花粉活力逐渐降低；除‘蝶衣重瓣’柱头无可授性外，其他品种开花当天柱头即具有可授性，第5～7 d柱头可授性最强，7 d后柱头可授性逐渐降低；‘蝶衣重瓣’בPKC’柱头上无花粉黏附，其余杂交组合授粉后父本花粉均能在母本柱头上萌发，存在不同程度花粉管缠绕与断裂现象，但最终都能到达子房与胚珠结合。更多还原

【Abstract】 To investigate the pollen viability, the changes in stigma receptivity, and the growth of pollen tubes after crossbreeding of different species of Cyclamen,and provide a foundation for hybrid breeding, 6 main cultivated varieties were used as materials.The pollen germination medium, the pollen viability and stigma receptivity of each Cyclamen variety in different days of blooming were tested, as well as the germination and growth of pollen tubes in three Reciprocal-hybrid combination were observed by fluorescence microscope.The results showed that the optimal pollen germination media for different species of Cyclamen differed significantly.Pollen viability varied in different flowering days, with‘Djix double petals’ having the strongest pollen viability at 2-3 d, and the rest of the Cyclamen varieties having high pollen viability at 2-5 d of flowering, followed by a gradual decrease after 5 d.Except for‘Djix double petals’,the stigmas of the other varieties were pollinated on the day of flowering.The stigmas were most pollinated in 5-7 d, and then gradually decreased after 7 d.There was no pollen adhesion on the stigma of‘Djix double petals’ בPKC’combination, and there were different degrees of pollen tube intertwine and fracture phenomena, but finally reached the ovary and ovule to complete fertilization.更多还原