【摘要】 GH3(Gretchen Hagen 3)基因家族属于植物生长素早期响应基因，与植物生长发育和逆境胁迫响应密切相关.基于三七全基因组数据，采用生物信息学分析方法，对三七GH3基因家族成员进行鉴定和分析，并通过RNA-seq和qRT-PCR技术分析了黑斑病菌胁迫下GH3家族基因成员的表达特征.结果显示，在三七基因组中共鉴定出15个GH3基因家族成员，其不均匀地分布于7条染色体上.系统进化树分析表明，PnGH3基因家族可以分为3个亚族，主要分布于亚族Ⅰ和Ⅲ.基因结构和保守基序分析表明，同一亚族具有类似的外显子/内含子数目和保守基序分布.启动子分析显示，PnGH3基因启动子区上主要包含4类顺式作用元件：光响应、植物激素响应、逆境胁迫响应和植物生长发育相关.共线性分析表明，三七GH3家族成员扩张主要源于片段重复事件，三七与木薯和拟南芥GH3基因家族成员之间具有较高的结构和功能相似性. RNA-seq和qRTPCR分析表明，PnGH3.1和PnGH3.9在黑斑病菌胁迫下显著上调表达（P<0.05），表明这些基因三七响应链格孢菌浸染过程中扮演着关键的角色.更多还原

【Abstract】 The GH3 gene family, known as an early responsive gene to auxin, is crucial in plant growth,development, and stress response. This study utilized bioinformatics analysis on P. notoginseng genome data to identify and analyze the members of the GH3 gene family. RNA-seq and qRT-PCR were employed to study the expression of PnGH3 family members under A. panax stress. The results revealed that 15 GH3 gene family members in the P. notoginseng genome, unevenly distributed across 7 chromosomes. Phylogenetic analysis classified the PnGH3 gene family into three subgroups, with predominant distribution in subgroups Ⅰ and Ⅲ.Gene structure and motif analysis indicated that the same subgroup had similar exon/intron number and conserved motif distribution. Promoter analysis highlighted the presence of cis-acting elements related to light response, plant hormone response, stress response, and plant growth and development. Collinearity analysis suggested that the expansion of P. notoginseng GH3 family members was primarily due to segmental duplication events, showing structural and functional similarities with GH3 gene family members of A. thaliana and M. esculenta. RNA-seq and qRT-PCR analysis demonstrated that PnGH3.1 and PnGH3.9 were significantly up-regulation under A. panax stress, which indicates their key role in P. notoginseng response to A. panax infection.更多还原