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玉米茎秆倒伏相关性状Meta-QTL分析及候选基因初步验证

Meta-QTL Analysis of Maize Stalk Lodging-Related Traits and Preliminary Validation of Candidate Genes

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【作者】 房海悦; 栾天宇; 王雪; 曲文利; 张春宵; 周小辉; 董菁; 李淑芳; 刘学岩; 李晓辉; 金峰学;

【Author】 FANG Hai-yue;LUAN Tan-yu;WANG Xue;QU Wen-li;ZHANG Chun-xiao;ZHOU Xiao-hui;DONG Jing;LI Shu-fang;LIU Xue-yan;LI Xiao-hui;JIN Feng-xue;Maize Research Institute, Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China);Qiqihar Sub-academy of Heilongjiang Academy of Agricultural Sciences;Foreign Experts Service Center of Jilin Province;

【通讯作者】 李晓辉;金峰学;

【机构】 吉林省农业科学院(中国农业科技东北创新中心); 黑龙江省农业科学院齐齐哈尔分院; 吉林省外国专家服务中心;

【摘要】 借助IBM2 2008 neighbors高密度遗传图谱整合802个玉米茎秆倒伏相关QTL,采用元分析方法获得64个MQTL(meta quantitative trait loci,MQTL),覆盖玉米全基因组。与水稻抗倒伏基因同源比对获得5个玉米茎秆倒伏相关候选基因,分别定位在玉米第1、3、4和9染色体上。通过基因表达分析表明,5个候选基因可能参与茎秆发育生物学过程,进而调控对茎秆机械强度。玉米茎秆倒伏相关性状MQTL及候选基因分析可以为分子标记辅助育种及相关基因克隆提供信息。

【Abstract】 Mining stalk lodging related genes is significant for maize variety improvement and molecular marker-assisted breeding(MAS). In this study, 64 meta-quantitative trait loci(MQTLs) were obtained by integrating 802maize stalk lodging related QTLs using the IBM2 2008 neighbors high-density genetic map, covering the entire maize genome. By comparing with rice cloned lodging orthologous genes, five candidate genes related to lodging resistance of maize stalk were obtained, located on maize chromosome 1, 3, 4, and 9, respectively. Gene expression analysis indicates that these 5 genes may be involved in the biological process of stalk development, thereby regulating the mechanical strength of the stalk. Analysis of MQTLs and related candidate genes for lodging related traits of maize stalk can provide useful information for molecular marker-assisted breeding and the cloning of related genes.

【基金】 吉林省农业科技创新工程项目“主要农作物种质资源鉴评及创制”(GXGC2024ZD003);科技创新2030-重大项目“养分高效高产玉米品种设计与培育”(2023ZD04067)
  • 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2025年08期
  • 【分类号】S513
  • 【下载频次】38
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