【摘要】 以抗、感自交系QB7866和QB5725为亲本构建的132份F2:3家系和包含142份温热玉米自交系组成的关联群体为材料，利用简化基因组测序技术和包含6万个SNP标记的MaizeSNP50芯片，分别对F2:3家系和关联群体进行基因型鉴定，对这两套群体的南方锈病抗性进行评价。结合连锁分析和全基因组关联分析，规模化鉴定控制玉米南方锈病抗性的遗传区段，并对相关候选基因进行预测。结果表明，共鉴定到13个玉米南方锈病抗性相关QTL，其中，贡献率大于10%的QTL两个。GWAS分析共检测到100个玉米南方锈病抗性相关QTNs。一致性分析发现3个一致性遗传区段。候选基因预测发现，这些区段内存在4个候选基因，可作为下一步精细定位的靶标。更多还原

【Abstract】 In this paper, 132 F_2:3 offspring derived from cross of susceptible corn inbred line QB7866 and resistant corn inbred line QB5725 and one associated population including 142 inbred lines were selected to be material,which were genotyped using genome by sequencing（GBS） and MaizeSNP50 chip with 60 000 SNPs, the resistance of these two populations to Southern corn rust was evaluated. By combining linkage analysis and genome-wide association analysis, lots of Southern corn rust resistance segments and related candidate genes were identified. The results showed that 13 QTL of the Southern corn rust were identified. Among these, 2 major QTL showed more than 10%contribution to the Southern corn rust. A total of 100 significant QTNs were detected by using GWAS method. Three concordant genetic segments were found by consistent analysis. Candidate gene prediction indicated the presence of 4 candidate genes within these segments, which can be target for further fine mapping.更多还原