【摘要】 目的：分析不同宫颈病变患者原癌基因（C-myc）、B细胞特异性莫洛尼白血病毒插入位点1(BMI-1)及阴道微生态[阴道pH值、乳酸杆菌、白细胞酯酶（LE）]表达水平，并探讨其与人乳头瘤病毒DNA(HPV DNA)载量的关系。方法：回顾性分析2022年1月—2023年1月海安市人民医院收治的不同宫颈病变患者的临床资料，其中包括80例子宫颈癌患者（子宫颈癌组）、96例宫颈上皮内瘤变患者（宫颈上皮内瘤变组），另选择65例宫颈正常患者作为宫颈正常组，比较三组患者的C-myc、BMI-1及阴道p H值、乳酸杆菌、LE、HPV DNA的水平范围，并采用Pearson相关分析验证不同宫颈病变患者C-myc、BMI-1以及阴道p H值、乳酸杆菌、LE与HPV DNA载量的相关性。结果：子宫颈癌组、宫颈上皮内瘤变组C-myc、BMI-1阳性细胞比例、阴道p H值、LE阳性比例及HPV DNA载量显著高于宫颈正常组，乳酸杆菌水平低于宫颈正常组，差异有统计学意义（P<0.05）。Pearson相关分析结果显示，C-myc、BMI-1阳性细胞比例、阴道pH值、LE阳性比例与HPV DNA载量均呈正相关，乳酸杆菌表达水平与HPV DNA载量均呈负相关（P<0.05）。结论：子宫颈癌及宫颈上皮内瘤变患者C-myc、BMI-1表达显著高于宫颈正常者，且与HPV DNA载量呈正相关，此外，相关阴道微生态失衡亦与HPV感染密切相关，其可能增加宫颈病变风险，因此在临床治疗过程中，应密切监测不同宫颈病变患者的C-myc、BMI-1、阴道微生态及HPV DNA载量水平变化，避免病情加重。更多还原

【Abstract】 Objective: To analyze the expression levels of proto-oncogene(C-myc), B-cell specific Moloni leukemia virus insertion site 1(BMI-1) and vaginal microecology [vaginal pH, lactobacillus, leucocyte esterase(LE)] in patients with different cervical lesions, and to explore the relationship between them and human papillomavirus DNA(HPV DNA) load. Method: Clinical data of patients with different cervical lesions admitted to Hai’an People’s Hospital from January 2022 to January 2023 were retrospectively analyzed, including 80 patients with cervical cancer(cervical cancer group) and 96 patients with cervical intraepithelial neoplasia(cervical intraepithelial neoplasia group), and 65 patients with normal cervix were selected as the normal cervix group. The levels of C-myc, BMI-1 and vaginal pH, lactobacillus, LE and HPV DNA in the three groups were compared, and Pearson correlation analysis was used to verify the correlation between C-myc, BMI-1, vaginal pH, lactobacillus, LE and HPV DNA load in patients with different cervical lesions. Result: The proportion of C-myc, BMI-1 positive cells, vaginal pH, LE positive proportion and HPV DNA load in cervical cancer group and cervical intraepithelial neoplasia group were significantly higher than those in normal cervical group, and the lactobacillus level was lower than that in normal cervical group, the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the proportion of C-myc, BMI-1 positive cells, vaginal pH value and LE positive proportion were positively correlated with HPV DNA load, while the expression level of lactobacillus was negatively correlated with HPV DNA load(P<0.05).Conclusion: The expressions of C-myc and BMI-1 in patients with cervical cancer and cervical intraepithelial neoplasia were significantly higher than those in normal cervical patients, and were positively correlated with HPV DNA load. In addition, the related vaginal microecological imbalance was also closely related to HPV infection, which may increase the risk of cervical lesions. Therefore, during clinical treatment, the changes of C-myc, BMI-1, vaginal microecology and HPV DNA load in patients with different cervical lesions should be closely monitored to avoid exacerbation of the disease.更多还原