【摘要】 为了探究热激转录因子（Hsf）在糙皮侧耳Pleurotus ostreatus胁迫响应以及生长发育中的功能，从糙皮侧耳基因组中鉴定出具有热激转录因子DNA结合结构域（DBD）的5个蛋白，命名为PoHsf1至PoHsf5。生物信息学分析结果显示，这5个蛋白均为不稳定亲水性蛋白，定位于细胞核。保守基序和系统进化分析发现，PoHsf1、PoHsf4与酿酒酵母Saccharomyces cerevisiae中的ScHsf1具有较近的亲缘关系，PoHsf2与ScSkn7高度相似。基因表达模式分析表明PoHsf1在原基期、子实体期以及高温胁迫下显著下调，而在低温和氧化胁迫下显著上调；PoHsf2和PoHsf3在原基期显著下调；PoHsf4在高温胁迫下显著上调；PoHsf5在原基期和子实体期显著上调。研究结果为糙皮侧耳热激转录因子家族成员的功能鉴定奠定了基础。更多还原

【Abstract】 To explore functions of heat shock transcription factors responsable to environmental stress and growth development of macrofungi,five proteins carrying the DNA binding domain of Hsf family were identified from Pleurotus ostreatus,as named as PoHsf1-PoHsf5,respectively.Bioinformatics analysis showed that all of the five PoHsfs belonged to unstable and hydrophilic proteins and were located in the cell nucleus.Detection of conserved motifs and phylogenetic analysis using the full protein sequences indicated that PoHsf1 and PoHsf4 were highly related to ScHsf1 while PoHsf2 was closely similar to ScSkn7 of Saccharomyces cerevisiae.Analysis of gene expression patters demonstrated that the activity of PoHsf1 was significantly downregulated during the two stages of primordial formation and fruiting body as well as under the high temperature stress but was significantly upregulated under the low temperature and hydrogen peroxide stress.The expression activity of both PoHsf2 and PoHsf3 was decreased during the primordial formation stage.Significant upregulation of exression activity was observed for PoHsf4 under the high temperature stress and for PoHsf5 during the primordial formation and fruiting body stages,respectively.The above study thus should provide a useful foundation for future understanding of the functional roles of heat shock transcription factors particularly on the stress responses in P.ostreatus.更多还原