【摘要】 半胱氨酸作为还原性生物硫醇,参与细胞内的氧化还原过程,在维持细胞稳态及生物体健康的过程中起着重要作用.研究表明,多种疾病的发生常伴随体内半胱氨酸含量的变化.因此,实时监测体内半胱氨酸含量的变化对于疾病的防治具有重要意义.传统生物样品中半胱氨酸的检测方法,测试条件要求高、生物相容性较差.基于荧光成像技术研发的荧光探针,具有特异性高、组织穿透性强、无创性等优点,可以实现实时定量监测生物体内的半胱氨酸.本综述总结了半胱氨酸荧光探针的构建策略、识别机理及其在医学检验领域中的应用,具体涉及到帕金森、阿尔兹海默病、肝脏损伤、关节炎、抑郁症和糖尿病等疾病,分析了在这些疾病发展过程中,荧光探针的信号响应及生物学应用,为人类健康和疾病研究提供更加精确的测定方法及诊断手段,同时也为荧光探针的开发提供新的思路和方法.更多还原

【Abstract】 As a reducing biothiol, cysteine participates in the redox process in cells and plays an important role in maintaining cellular homeostasis and organismal health. Previous studies showed that the occurrence of many diseases was often accompanied by changes in the level of cysteine in the body. Therefore, real-time monitoring of changes in cysteine content in the body is of great significance for the prevention and treatment of diseases. Traditional methods for the detection of cysteine in biol ogical samples have strict test conditions and poor biocompatibility. The fluorescent probe developed based on fluorescence imaging technology possesses the advantages of high specificity, strong tissue penetration and non-invasiveness, and can recognize real-time quantitative monitoring of cysteine in organisms. In this review, the construction strategies, recognition mechanisms and applications of cysteine fluorescent probes in the field of medical testing are summarized, specifically involving Parkinson’s disease, Alzheimer’s disease, liver injury, arthritis, depression and diabetes, and the signal response and biological applications of fluorescent probes in the development of these diseases are analyzed. It will provide more accurate measurement methods and diagnostic methods for human health and disease research, and also provide new ideas and methods for the development of fluorescent probes.更多还原