【摘要】 为了探讨熊胆止痛凝胶剂的抗炎镇痛作用及其可能的作用机制,采用冰醋酸扭体法、二甲苯致耳肿胀法和角叉菜胶致足肿胀法,评估了该熊胆止痛凝胶剂的抗炎镇痛效果.采用酶联免疫法测定了大鼠左后侧足跖组织中IL-6、IL-1β及PGE2的水平,使用Western blot法测定组织中TNF-α、COX-2的蛋白表达.研究结果显示:与模型组相比,熊胆止痛凝胶剂的低、中、高剂量组均能够减少小鼠的扭体次数,延长扭体潜伏期,并呈现剂量依赖性;与模型组相比,熊胆止痛凝胶剂的各剂量组均可显著降低由二甲苯所致的耳肿胀度和角叉菜胶所致的足肿胀度.此外,熊胆止痛凝胶剂还可以降低大鼠左后侧足跖组织中IL-6、IL-1β和PGE2的含量,以及显著降低TNF-α和COX-2的蛋白表达水平,且呈现剂量依赖性.上述研究结果初步表明,熊胆止痛凝胶剂具有一定的镇痛和抗炎作用,其机制可能与降低TNF-α和COX-2的表达来改善炎症反应有关.更多还原

【Abstract】 To investigate the anti-inflammatory and analgesic effects of bear bile analgesic gel(XBD) and its possible mechanism of action, the self-made XBD were evaluated using the acetic acid writhing method, xylene-induced ear swelling method, and carrageenan-induced paw swelling method.The levels of IL-6, IL-1β, and PGE2 in the left hind paw tissue of rats were determined by enzyme-linked immunosorbent assay, and the protein expression of TNF-α and COX-2 in the tissue was determined by Western blot.The results showed that compared with the model group, the low, medium, and high-dose groups of XBD could reduce the number of writhing in mice, prolong the writhing latency, and show a dose-dependent effect.Meantime, compared with the model group, the degrees of the xylene-induced ear swelling and carrageenan-induced paw swelling in each dose group of XBD were significantly reduced.In addition, XBD could also reduce the levels of IL-6, TNF-α, and PGE2 in the left hind paw tissue of rats.Western blot results showed that compared with the model group, each dose of XBD could significantly reduce the protein expression levels of TNF-α and COX-2, showing a dose-dependent effect.The results preliminarily indicated that XBD had certain analgesic and anti-inflammatory effects, and its mechanism may be to improve the inflammatory response by reducing the expression of TNF-α and COX-2.更多还原