【摘要】 目的 探讨蛋白激酶cAMP激活催化亚基β(PRKACB)基因在泛癌组织中的转录组及蛋白表达特征，并分析其表达水平与临床预后及肿瘤微环境免疫浸润的相关性。方法 基于癌症基因组图谱（TCGA）、临床蛋白质组学肿瘤分析联盟（CPTAC）和基因表达综合数据库（GEO）等多组学数据，系统分析PRKACB在33种恶性肿瘤中的表达差异、生存预后、体细胞突变及免疫细胞浸润特征。结果 PRKACB基因在膀胱尿路上皮癌（BLCA）、乳腺癌（BRCA）、子宫颈癌（CESC）等多种恶性肿瘤中呈现显著差异性表达，且其表达水平与患者预后显著相关。PRKACB突变主要包括错义突变、无义突变、剪接位点突变和基因融合，具有明显的肿瘤异质性。免疫浸润分析表明，PRKACB基因表达水平与CD8~+T细胞浸润程度在不同肿瘤类型中呈现显著异质性。相关基因富集分析揭示，PRKACB基因可能通过影响细胞信号传导、细胞周期调控及免疫细胞浸润参与肿瘤的发生和发展过程。结论 PRKACB基因在多种肿瘤中具有复杂的生物学作用，其表达水平和突变特征与患者预后及免疫微环境密切相关，有望成为潜在的预后标志物或治疗靶点。更多还原

【Abstract】 Objective To investigate the transcriptome and protein expression characteristics of protein kinase cAMP-activated catalytic subunit β(PRKACB) gene in pan-cancerous tissues,and to analyze the correlation between its expression level and clinical prognosis and tumor microenvironment immune infiltration.Methods Based on multi-omics data such as Cancer Genome Atlas(TCGA),Clinical Proteomics Cancer Analysis Consortium(CPTAC)and Gene Expression Omnibus(GEO),the expression differences,survival prognosis,somatic mutations and immune cell infiltration characteristics of PRKACB in 33 malignant tumors were systematically analyzed.Results PRKACB gene showed significant differential expression in bladder urothelial carcinoma(BLCA),breast cancer(BRCA),cervical cancer(CESC) and other malignant tumors,and its expression level was significantly correlated with the prognosis of patients.PRKACB mutation mainly includes missense mutation nonsense mutation splicing site mutation and gene fusion which has obvious tumor heterogeneity.Immune infiltration analysis showed that the expression level of PRKACB gene and the degree of CD8+T cell infiltration showed significant heterogeneity in different tumor types.The enrichment analysis of related genes revealed that PRKACB gene may be involved in the occurrence and development of tumors by affecting cell signal transduction,cell cycle regulation and immune cell infiltration.Conclusion PRKACB gene has complex biological functions in a variety of tumors.Its expression level and mutation characteristics are closely related to the prognosis and immune microenvironment of patients,and it is expected to become a potential progno stic marker or therapeutic target.更多还原