【摘要】 目的 探究杜仲叶和栀子提取物对白色脂肪细胞褐变的促进作用，并探究其可能的机制。方法 采用CCK8法、Western blotting法、qRT-PCR法、油红O染色法、BODIPY染色法、线粒体荧光染料检测杜仲叶和栀子提取物对成熟的3T3-L1细胞褐变的影响，采用自噬激活剂（雷帕霉素）探明其可能的作用机制。结果 研究显示杜仲叶和栀子提取物能够上调褐变相关蛋白及mRNA(PRDM16、PGC-1α、UCP-1)的表达（P均<0.05），抑制自噬相关蛋白（Beclin-1、p62）和mRNA(LC3、Beclin-1、p62)及自噬小体的表达（P均<0.05），抑制脂滴积累，增加线粒体丰度。采用自噬激活剂（雷帕霉素）恢复杜仲叶和栀子提取物对3T3-L1细胞自噬水平后（P均<0.05），发现杜仲叶和栀子提取物对3T3-L1细胞的褐变作用也被自噬激活剂逆转（P均<0.05）。结论 杜仲叶和栀子提取物可能通过抑制自噬进而促进白色脂肪细胞褐变，其在临床防治肥胖及相关代谢性疾病方面具有潜在作用。更多还原

【Abstract】 Objective To investigate whether extracts of Eucommia ulmoides leaves and Gardenia have the effects on promoting white adipose browning, and to explore their possible mechanisms. Methods The effects of Eucommia ulmoides leaves and Gardenia extracts on mature 3T3-L1 cells were detected using CCK8 assay, Western blotting, qRT-PCR, Oil red O staining, BODIPY staining, and mitochondrial fluorescent dye. Subsequently, autophagy inhibitors(rapamycin) were used to explore their possible mechanisms of action. Results Eucommia ulmoides leaves and Gardenia extracts could upregulate browning-related proteins and mRNAs(PRDM16, PGC-1α and UCP-1)(all P < 0.05), inhibited autophagy-related proteins(Beclin-1 and p62) and mRNAs(LC3, Beclin-1 and p62) and the expression of autophagosomes(all P < 0.05), inhibited lipid droplet accumulation, and increased mitochondrial abundance. The use of autophagy activator(rapamycin) restored the autophagy effect of Eucommia ulmoides leaf and Gardenia extracts on 3T3-L1 cells(all P < 0.05), and it was found that the browning effect of Eucommia ulmoides leaf and Gardenia extracts on 3T3-L1 cells was also reversed by autophagy activator(all P < 0.05). Conclusion Extracts of Eucommia ulmoides leaves and Gardenia have the effects upon promoting the browning of white adipocytes by inhibiting autophagy, demonstrating potential clinical applications in the clinical prevention and treatment of obesity and related metabolic diseases.更多还原