【摘要】 目的:对比分析温度循环保存血小板与4℃冷藏保存、22℃振荡保存血小板的聚集率及血小板常规参数变化，为探讨血小板保存方法提供更多实验数据。方法:对温度循环保存血小板、4℃冷藏保存血小板、22℃振荡保存血小板保存d 1、2、3、4、6共5个时间点采集血样，检测其血小板最大聚集率(MAR)及血小板常规参数，包括血小板计数(PLT)、平均血小板体积(MPV)、血小板分布宽度(PDW)和大血小板比率(P-LCR)。结果:3组血小板MAR均随保存时间延长呈显著下降趋势，以22℃组下降最快，4℃组最慢，温度循环组介于两者之间；保存d 3,4℃组MAR仍在正常范围内(MAR>60%),温度循环组约50%,22℃组最低；保存d 4,3组MAR均低于50%,温度循环组MAR显著低于4℃组且高于22℃组(均P<0.05);保存d 6,温度循环组MAR显著低于4℃组(P<0.05),而与22℃组差异无统计学意义(P>0.05)。3组PLT值均随保存时间延长显著下降，保存6 d内3组PLT值均显著低于保存初期(均P<0.05);温度循环组6 d内PDW无显著变化，MPV、P-LCR显著升高；4℃组6 d内MPV、PDW、P-LCR均显著下降，而22℃组显著上升；在相同保存天数下温度循环组PLT值与4℃组、22℃组无明显差异，MPV、PDW、P-LCR值显著高于4℃组、低于22℃组(均P<0.05)。结论:温度循环保存血小板的聚集功能、血小板常规参数变化介于4和22℃保存之间。更多还原

【Abstract】 Objective: To compare and analyze the changes of aggregation rate and routine parameters of platelets stored in temperature cycle, cold storage at 4 ℃ and oscillating storage at 22 ℃, so as to provide more experimental data for platelet preservation methods. Methods: Blood samples were collected at 5 time points on the 1^st, 2^nd, 3^rd, 4^th and 6^th day after platelet cycling preservation at temperature, cold storage at 4 ℃, and oscillating storage at 22 ℃. Platelet maximum aggregation rate（MAR） and routine parameters including platelet count（PLT）, mean platelet volume（MPV）, platelet distribution width（PDW） and platelet-larger cell ratio（P-LCR） were detected. Results: The platelet MAR of three groups showed a significant decrease trend with the preservation time, the fastest decrease was in the 22 ℃ group, the slowest was in the 4 ℃ group, and the temperature cycle group was between the two groups. On the 3^rd day of preservation, the platelet MAR in 4 ℃ group was still in the normal range（MAR>60%）, while in temperature cycle group was about 50%, and in 22 ℃ group was the lowest. On the 4^th day of preservation, platelet MAR in all the three groups was lower than 50%, and that in temperature cycle group was significantly lower than in 4 ℃ group but higher than in 22 ℃ group（both P<0.05）. On the 6^th day of preservation, platelet MAR in the temperature cycle group was significantly lower than that in the 4 ℃ group（P<0.05）, but there was no statistically significant difference compared to 22 ℃ group（P>0.05）. PLT values in the three groups were all significantly decreased with the preservation time extension, and were significantly lower than those in the early stage of preservation within 6 days（all P<0.05）. PDW in temperature cycle group had no significant change within 6 days of preservation, but MPV and P-LCR were significantly increased. MPV, PDW and P-LCR all decreased significantly in 4 ℃ group within 6 days of preservation but increased in 22 ℃ group. Under the same storage days, PLT value of temperature cycle group had no significant difference with that of 4 ℃ group and 22 ℃ group, while MPV, PDW and P-LCR values were significantly higher than 4 ℃ group but lower than 22 ℃ group（all P<0.05）. Conclusion: The aggregation function and routine parameters changes of temperature circulating preserved platelets are between 4 and 22℃.更多还原