【摘要】 目的:筛选多发性骨髓瘤(MM)的新型诊断标志或治疗靶点。方法:应用生物信息学方法，基于GEO数据库筛选出Sel1L、SPAG4、KCNN3和PARM1 4个MM高表达分子，采用RT-PCR和Western blot方法检测骨髓瘤细胞系U266、NCI-H929、MM.1s、RPMI8226和白血病细胞系THP1及31例MM患者骨髓单个核细胞中这4种分子的RNA和蛋白表达水平，同时用5例异基因移植健康供者的骨髓标本作对照。结果:与白血病细胞系THP1相比，KCNN3、PARM1、Sel1L在U266、NCI-H929、MM.1s中的表达水平较高，而PARM1在8226中的表达水平更高；Western blot结果显示，这4种基因在4种骨髓瘤细胞系中均有蛋白表达。与健康供者相比，Sel1L、SPAG4、KCNN3和PARM1 mRNA在MM患者中的表达水平更高(均P<0.05);Western blot结果显示，这4种基因在MM患者中均有蛋白表达，且Sel1L、KCNN3水平与健康供者相比差异具有统计学意义(均P<0.01)。结论:Sel1L、SPAG4、KCNN3和PARM1有可能成为MM的诊断标志和治疗靶点。更多还原

【Abstract】 Objective: To screen novel diagnostic marker or therapeutic target for multiple myeloma(MM). Methods: Sel1L, SPAG4, KCNN3 and PARM1 were identified by bioinformatics method based on GEO database as high expression genes in MM. Their RNA and protein expression levels in bone marrow mononuclear cells from myeloma cell lines U266, NCI-H929, MM.1s, RPMI8226 and leukemia cell line THP1, as well as 31 MM patients were evaluated by RT-PCR and Western blot, respectively. Meanwhile, 5 samples of bone marrow from healthy donors for allogeneic hematopoietic stem cell transplantation were employed as controls. Results: Compared with leukemia cell line THP1, the expression levels of KCNN3, PARM1 and Sel1L mRNA were significantly increased in myeloma cell lines U266, NCI-H929 and MM.1s, while PARM1 was further increased in myeloma cell lines 8226. Western blot showed that the 4 genes were all expressed in the 4 myeloma cell lines. Compared with healthy controls, the expression levels of Sel1L, SPAG4, KCNN3 and PARM1 mRNA were significantly higher in MM patients(all P<0.05). Western blot showed that the 4 genes were all expressed in MM patients, and the protein expression level of Sel1L and KCNN3 were significantly different compared with healthy donors(all P<0.01). Conclusion: Sel1L, SPAG4, KCNN3 and PARM1 may be potential diagnostic markers and therapeutic targets for MM.更多还原