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白翎芽孢杆菌耐盐碱促生潜力评价分析
Evaluation and Analysis of the Growth-Promoting Potential of Bacillus Baekryungensis Resistant to Salt and Alkali
【摘要】 本文研究旨在盐胁迫下白翎芽孢杆菌定殖对绿豆和水稻生长的影响。在本研究中,白翎芽孢杆菌具有较高耐盐碱能力,能在盐浓度8%和pH10中仍可存活。白翎芽孢杆菌有较高的产IAA能力(5.26±0.03μg/mL),,1-氨基环丙烷-1-羧酸活性酶值为0.0157±0.512U/mg,以促进植物在盐胁迫下生长,具有溶无机磷(SI=D/d=1.21±0.01),溶有机磷(SI=D/d=3.17±0.02),以及解钾(4.82±0.68)能力,从而抑制NaCl的摄入,减轻了盐离子对植物体的伤害。定殖规律表明,在定殖组里,白翎芽孢杆菌均为优势菌群,除水稻叶子外定殖率为30%以外,水稻其他部位和绿豆的不同部位的定殖率均高于50%以上。定殖组和对照组的生长指标的测定结果表明,白翎芽孢杆菌的定殖有利于盐胁迫下的植物生长,减轻植物体内丙二醛伤害,对照组的SOD和POD酶活性均高于定殖组。
【Abstract】 This study aims to investigate the effects of Bacillus albicans colonization on the growth of mung bean and rice under salt stress. In this study, B. baekryungensis had high salinity tolerance and could survive in salt concentration of 8% and PH10. B. baekryungensis has high IAA(5.26±0.03 μg/mL), production and 1-a minocyclopmpane-1-carboxylate activity activity with a value of 0.0157 ± 0.512 U/mg, which could promote plant growth under salt stress. It also has the ability to dissolve inorganic phosphorus(SI = D/d = 1.21 ± 0.01), dissolve organic phosphorus(SI = D/d = 3.17 ± 0.02), dissolve phosphorus and release potassium(4.82 ± 0.68), thereby inhibiting the intake of NaCl and reducing the damage of salt ions to plants. The colonization pattern showed that B. baekryungensis was the dominant bacterial group in all the colonized groups, except for the colonization rate of 30% on rice leaves, the colonization rates on other parts of Oryza sativa and different parts of Vigna radiata were all above 50%. The growth indexes of the colonization group and the control group showed that the colonization of B. baekryungensis was beneficial to the growth of plants under salt stress and reduced the damage of malondialdehyde in the plants, and the activities of SOD and POD in the control group were higher than those in the colonization group.
【Key words】 Bacillus baekryungensis; salt stress; Oryza sativa; Vigna radiate; plant growth promoting bacteria;
- 【文献出处】 信阳农林学院学报 ,Journal of Xinyang Agriculture and Forestry University , 编辑部邮箱 ,2025年02期
- 【分类号】S156.4
- 【下载频次】16