【摘要】 目的 探讨热休克蛋白70(HSP70)家族基因多态性及其血清蛋白与职业心理应激交互作用对转氨酶活性的影响。方法 采用病例-对照研究，选取无血缘关系的583例代谢综合征（MS）患者为病例组、585名健康人为对照组。进行职业心理应激等问卷调查、体格检查、临床以及生化指标测定、血清HSP70水平以及HSP70基因5位点多态性检测；应用Logistic回归分析HSP70基因5位点多态性及其血清蛋白与职业心理应激交互作用对转氨酶活性的影响。结果 Logistic回归分析结果显示：调整MS、年龄、性别、婚姻状况、文化程度、吸烟以及饮酒因素后，应激水平与rs1008438位点的低应激CC、高应激AA、高应激AC交互组合，与rs1043618位点的低应激CC、高应激GG、高应激GC交互组合，与rs539689位点的高应激CC交互组合对天门冬氨酸氨基转移酶（AST）影响均有统计学意义（P均<0.05）；应激水平组与rs1061581位点的低应激AG、低应激GG、中应激GG、中应激AA交互组合对AST影响均有统计学意义（P均<0.05）。应激水平组与rs1008438位点的低应激CC、中应激AC、高应激AA、高应激AC交互组合，与rs1043618位点的低应激CC、高应激GG、高应激GC交互组合对丙氨酸氨基转移酶（ALT）影响均有统计学意义（P均<0.05）；应激水平组与rs1061581位点的低应激AG、低应激GG、中应激GG、中应激AA交互组合，与rs539689位点的中应激GG交互组合对ALT影响均有统计学意义（P均<0.05）。结论 HSP70家族基因与职业心理应激交互作用可影响血清AST、ALT水平，不同应激水平与突变纯合、杂合或野生纯合子交互作用可使转氨酶活性增高的风险上升或降低。更多还原

【Abstract】 Objective To explore the effecs of HSP70 protein family gene polymorphism and the interaction between serum protein and occupational psychological stress on transaminase activity. Methods Using a case-control study,based on inclusion and exclusion criteria,583 unrelated MS patients were selected as the case group and 585 healthy individuals as the control group. Questionnaire survey on occupational psychological stress,physical examinations,clinical and biochemical indicators,serum HSP70 level and HSP70 gene 5 locus polymorphism were detected;Logistic regression was used to analyze the effect of HSP70 gene five locus polymorphism and the interaction between serum protein and occupational psychological stress on transaminase activity. Results Logistic regression analysis showed that after adjusting for factors such as MS,age,gender,marriage,education level,smoking,and alcohol consumption,the stress level group had a significant impact on AST with low stressCC,high stressAA,and high stressAC interaction combinations at rs1008438,low stressCC,high stressGG,and high stressGC interaction combinations at rs1043618,and high stressCC interaction combinations at rs539689(P<0.05);The interaction combination of low stressAG,low stressGG,moderate stressGG,and moderate stressAA at the rs1061581 locus with the stress level group had a significant impact on AST(P<0.05). The interaction combinations of low stressCC,medium stressAC,high stressAA,and high stressAC at rs1008438 and low stressCC,high stressGG,and high stressGC at rs1043618 had significant effects on ALT(P<0.05);The stress level group and the low stress AG,low stressGG,medium stressGG,and medium stressAA interaction combinations at rs1061581 locus,as well as the medium stressGG interaction combination at rs539689 locus,all had significant effects on ALT(P<0.05). Conclusion The interaction between HSP70 family genes and occupational psychological stress can affect serum AST and ALT levels. The interaction between different stress levels and mutant homozygotes,heterozygotes or wild homozygote can increase or decrease the risk of increased transaminase activity.更多还原