【摘要】 旨在探究猪肌肉发育的分子基础，基于前期进行的泛素化蛋白组学测序(4D-label泛素化测序)，检测杜洛克猪和藏香猪腿肌样本的翻译后水平修饰，分析两品种猪骨骼肌中编码泛素化底物蛋白的锌指C3H1型蛋白(ZFC3H1)的差异性基因。通过UniPort、GeneCards、Ensembl数据库查询ZFC3H1蛋白质序列并进行生物信息学分析，使用PLAAC与PONDR进行相分离能力预测，最后利用ISwine和PigGTEx统计与脂肪发育及产肉性状相关的数量性状基因座(QTLs)的数量关系。结果:ZFC3H1蛋白为亲水蛋白，含有411个磷酸化位点及14个糖基化位点，不存在信号肽及跨膜区域，主要分布在细胞核上，二级结构预测结果显示α-螺旋、无规卷曲和延伸链分别占37.16%、62.33%、0.51%，与三级结构预测一致; ZFC3H1与微管相关蛋白TREX复合体亚基(MTREX)、PNN互作的丝氨酸/精氨酸富集蛋白(PNISR)、含锌指CCHC结构域蛋白7(ZCCHC7)、细胞色素c氧化酶组装因子(CMC1)、多聚腺苷酸结合蛋白核1(PABPN1)等蛋白之间存在互作;系统进化树显示，该基因与非洲野猪亲缘关系最近，同源性为99.8%;相分离能力预测及QTLs统计显示，ZFC3H1的蛋白质结构中存在类朊病毒结构的蛋白序列和9个内在无序区域(IDRs)，同时该基因含有10个有价值的表达数量性状基因座(e QTLs)，且基因附近有218个长链非编码RNA表达数量性状基因座(lncQTLs)。结论:本试验成功证明ZFC3H1可形成生物凝聚体并驱动液-液相分离，进而调控骨骼肌增殖。更多还原

【Abstract】 The aim of this study was to delve into the molecular basis of porcine muscle development. Based on the previous ubiquitination proteomics sequencing( 4D-label ubiquitination sequencing),the post-translational modifications of leg muscle samples from Duroc pigs and Tibetan pigs were detected,and the differential genes encoding the ubiquitinated substrate protein-zinc finger C3H1-type protein( ZFC3H1) in the skeletal muscles of the two pig breeds were analyzed. Then,the protein sequence of ZFC3H1 was queried using Uni Port,Gene Cards and Ensembl databases for bioinformatics analysis. Next,PLAAC and PONDR were used to predict the phase separation ability.Finally,ISwine and PigGTEx were utilized to count the quantitative relationship between QTLs related to fat and meat production traits. The results showed that ZFC3H1 was a hydrophilic protein,containing 411 phosphorylation sites and 14 glycosylation sites,with no signal peptide or transmembrane region,and it was mainly distributed in the nucleus. The secondary structure prediction results indicated that α-helix,random coil,and extended chain account for 37. 16%,62. 33%,and 0. 51%,respectively; which was consistent with the tertiary structure prediction. ZFC3H1 interacted with proteins such as icrotubule-associated protein TREX complex subunit( MTREX),peptidylprolyl cistrans isomerase N1 domain-containing protein( PNISR),zinc finger CCHC domain-containing protein 7( ZCCHC7),cytochrome c oxidase assembly factor( CMC1),and polyadenylate-binding protein nuclear 1( PABPN1). The phylogenetic tree showed that this gene had the closest genetic relationship with the African wild boar,with a homology of 99. 8%. The phase separation ability prediction and QTLs statistics showed that the protein structure of ZFC3H1 contained prion-like protein sequences and 9 intrinsically disordered regions( IDRs). Meanwhile,this gene contained 10 valuable expression quantitative trait loci( e QTLs),and there were 218 long non-coding RNA expression quantitative trait loci( lnc QTLs) near the gene. It was successfully proven here that the effector ZFC3H1 was able to form biological condensates and to drive liquid-liquid phase separation so as to regulate skeletal muscle proliferation.更多还原