【摘要】 旨在研究不同滴度猪流行性腹泻病毒（PEDV）感染对断奶仔猪行为模式的影响。选取24只21日龄的三元杂交断奶仔猪，随机分为对照（CON）组、低剂量病毒（LDV）组和高剂量病毒（HDV）组，每组单独猪舍饲养，适应饲养7 d后于28日龄进行灌喂，CON组灌喂1 mL DMEM培养基，LDV组灌喂1 mL滴度为5×10⁵ TCID₅₀/mL的病毒液，HDV组灌喂1 mL滴度为1×10⁷ TCID₅₀/mL的病毒液。试验为期7 d,期间通过摄像头记录并储存行为视频数据。结果：CON组所有断奶仔猪均呈现抗体阴性，LDV组断奶仔猪抗体阳性比例为37.5%,HDV组断奶仔猪抗体阳性比例为50%,LDV和HDV组腹泻仔猪头数较CON组有所增加。在个体水平上，LDV和HDV组的活动时长和探索时长较CON组显著减少（P<0.05）,LDV组采食频率显著下降，HDV组积极社交时长较CON组显著减少（P<0.01）。在群体水平上，HDV组群体积极社交时长显著减少（P<0.01）,活动时长和探索时长也有减少的趋势，LDV组群体采食频率较CON组显著下降（P<0.05）。LDV和HDV感染组采食模式发生改变，并在感染后第6天趋于正常。在感染前期，尤其是上午8:30—10:30时段，LDV和HDV组的活动时长、探索时长和积极社交时长较CON组有不同程度的减少。LDV和HDV组离群频率在感染后较CON组增加并在感染第6天恢复正常。综上，本试验初步将活动行为、探索行为和积极社交行为视为早期疾病预警的关键行为，且在第一次饲喂后2 h群体行为变化尤为明显。更多还原

【Abstract】 To study the effect of different titers of PEDV infection on the behavior pattern of weaned piglets,twenty-four 21-day-old weaned ternary hybrid piglets were selected in this study,and they were randomly allocated to three groups:the CON group,the LDV group,and the HDV group.Each group was housed in a separate pen,and they were fed at 28 days of age after 7 days of adaptation,with 1 ml of DMEM （cell culture medium） in the CON group,1 ml G2c PEDV strain with a titer of 5×10⁵TCID₅₀/ml in the LDV group,and 1 ml G2c PEDV strain with a titer of 1×10⁷TCID₅₀/ml in the HDV group.The experiment lasted for 7 days,during which the video data of the behavior was recorded and stored by the camera.The results were as follows:All weaned piglets in the CON group were antibody-negative,the proportion of the weaned piglets in the LDV group was 37.5%,that of weaned piglets in the HDV group was 50%,and the number of piglets with diarrhea in the infected group increased,as compared with that in the CON group.At the individual level,the duration of moving and pen exploration time of the LDV and HDV infection group were significantly lower than those of the CON group （P<0.05）.Compared with the CON group,the feeding frequency of the LDV group decreased significantly,but the duration of positive socializing in the HDV group was significantly lower （P<0.01）.At the group level,the duration of positive socializing in the HDV group was significantly reduced （P<0.01）,the duration of moving and pen exploration were also reduced,and the eating frequency in the LDV group was significantly lower than that in the CON group （P<0.05）.The eating pattern of the LDV and HDV infected group changed and tended to get normal on the 6th day after infection.In the early stage of infection,especially in the 8:30-10:30 a.m.period,the duration of moving,pen exploration and positive socializing of the LDV and HDV infected group decreased to varying degrees,compared with that of the CON group.The outlier frequency of the LDV and HDV infected group increased,compared with the CON group after infection and returned to normal on the 6th day of infection.Taken together,in this study,moving,pen exploration and positive social behavior were initially regarded as the key behaviors for early disease warning,and the changes in various behaviors were particularly obvious 2 h after the first feeding.更多还原