【摘要】 目的：探究萝卜硫素（SFN）对小鼠缺氧耐受力的影响.方法：通过常压密闭、垂体后叶素注射液皮下注射及中毒剂量亚硝酸钠（NaNO₂）腹腔注射分别构建乏氧性（低张性）缺氧、心肌缺血缺氧损伤及高铁血红蛋白血症缺氧三种常用实验性缺氧模型，观察SFN对不同缺氧模型小鼠生存时间的影响，评价SFN提高小鼠缺氧代偿能力的药理作用.结果：SFN低、中、高（SFN-L,SFN-M,SFN-H）剂量组均能延长三种缺氧模型小鼠生存时间.其中与生理盐水对照组比较，SFN-L、SFN-M、SFN-H组均能显著延长乏氧性缺氧小鼠生存时间；与垂体后叶素模型组比较，SFN-L、SFN-M、SFN-H组均能显著延长心肌缺血缺氧损伤小鼠生存时间；与NaNO₂模型组比较，SFN-M、SFN-H组能显著延长高铁血红蛋白血症缺氧小鼠生存时间，均有显著性差异（P<0.05或P<0.01），表明SFN能提高三种模型小鼠对缺氧的代偿能力.结论：在体条件下，SFN具有抗缺氧作用，其抗缺氧机制可能与SFN抗氧化特性有一定相关性.更多还原

【Abstract】 ObjectiveTo explore the effect of sulforaphane（SFN）on hypoxia tolerance in mice. Methods Three commonly used experimental hypoxia models were established as follows including the normobaric closed environment for hypoxic（hypotonic） hypoxia,subcutaneous injection of pituitrin for myocardial ischemic hypoxia injury,and intraperitoneal injection of a toxic dose of sodium nitrite（NaNO₂）for methemoglobinemia-induced hypoxia. The effects of SFN on the survival time of mice were observed in the different hypoxia models,and the pharmacological actions of SFN on enhancing hypoxia compensatory capacity of mice were evaluated. Results The result showed three SFN groups（SFN-L,SFN-M and SFN-H）extended the survival time of mice in all three hypoxia models. Specifically,（1）compared with the normal saline control group,SFN-L,SFN-M and SFN-H all markedly prolonged survival time in hypoxic hypoxia model mice；（2）relative to the pituitrin model group,SFN-L,SFN-M and SFN-H significantly increased survival duration in myocardial ischemic hypoxia injury mice；（3）in comparison with the NaNO2 model group,SFN-M and SFN-H demonstrated significant extension of survival time in methemoglobinemia-induced hypoxic mice. All these effects showed statistically significant differences（P<0.05 or P<0.01）,indicating that SFN can enhance compensatory capacity against hypoxia in all three model systems. Conclusions In vivo,SFN exhibited anti-hypoxic effects,and its antihypoxic mechanism may be associated with the antioxidant properties of SFN.更多还原