【摘要】 【目的】番茄褪绿病毒（Tomato chlorosis virus, ToCV）能够侵染番茄、辣椒和茄子等茄果类蔬菜，造成产量和品质下降。探明采集自新疆库尔勒市英下乡设施蔬菜种植区茄子病毒病的种类，将为该病害的防治奠定理论基础。【方法】使用ToCV、TSWV、TMV和ToBRFV病毒的特异引物对茄子样品中病毒的种类进行分子鉴定，并对扩增得到的序列使用最大似然法进行系统进化分析。【结果】仅有ToCV检测引物可以扩增出431 bp特异性条带；经测序，该序列与ToCV序列（KR072214）相似性最高，为99%，表明茄子样品中的病毒为ToCV；系统进化分析表明，本研究获得的ToCV茄子分离物与天津（KP893120）、山东（KP072214）、西班牙（AAZ77648）、巴西利亚（AFN44466）、希腊（ABX89240）的ToCV分离物聚为一支，与天津ToCV分离物的亲缘关系最近。【结论】证实番茄褪绿病毒在新疆库尔勒市英下乡设施市茄子上发生危害，未与ToCV、TSWV、TMV和ToBRFV等病毒复合侵染。更多还原

【Abstract】 【Objective】 Tomato chlorosis virus(ToCV) can infect solanaceous vegetables such as tomato, pepper and eggplant, resulting in a decrease in yield and quality. This project aims to find out the types of eggplant virus diseases collected from the greenhouse vegetable planting area in Yingxia Township,Korla City, Xinjiang in the hope of laying a theoretical foundation for the prevention and control of the disease.【Methods】 The specific primers of ToCV, TSWV, TMV and ToBRFV viruses were used to identify the virus species in eggplant samples, and the phylogenetic analysis of the amplified sequences was carried out by using the maximum likelihood method. 【Results】The specific band of 431 bp could be amplified only by the detection primers of ToCV. After sequencing, the sequence had the highest similarity with the ToCV sequence(KR072214), which was 99%, indicating that the virus in the eggplant sample was ToCV; Phylogenetic analysis showed that the ToCV eggplant isolates obtained in this study were clustered with ToCV isolates from Tianjin(KP893120), Shandong(KP072214), Spain(AAZ77648), Brasilia(AFN44466) and Greece(ABX89240), and afterwards, the phylogenetic relationship with ToCV isolates from Tianjin was analyzed.【Conclusion】 It is confirmed for that tomato chlorosis virus is harmful to facility eggplants in Yingxia Township, Korla City, Xinjiang, and it was not co-infected with ToCV, TSWV,TMV and ToBRFV.更多还原