【摘要】 【目的】为研究不同植物激素对红花再生体系的影响。【方法】以新红花4号叶片为外植体，利用不同植物激素配比对外植体进行培养。【结果】诱导红花叶片成愈伤组织的最佳培养基为MS+6-BA 3 mg/L+NAA 0.5 mg/L，愈伤率达91.3%。培养20 d后，诱导叶片愈伤组织分化不定芽的最佳培养基为MS+KT 3.0 mg/L+IAA 0.05 mg/L。培养30 d后，最佳继代增殖培养基为MS+KT 3.0 mg/L+IAA 0.1 mg/L，增殖系数可达111.7。瓶内生根最佳培养基为MS+NAA 0.5 mg/L+IBA 0.9 mg/L，生根总数达43个，生根率达90.9%。【结论】建立了愈伤诱导、不定芽分化、瓶内生根的60 d红花再生体系，为红花生物育种提供技术支撑。更多还原

【Abstract】 【Objective】To study the effects of phytohormones on the rapid propagation and rooting of safflower, with a view to constructing a complete system of safflower primary culture, secondary proliferation,and rooting in vials. 【Methods】Saffron tissue culture was carried out with the new Saffron 4 leaf explants as the test material, and the organogenesis type was adopted as the main culture pathway. 【Results】The optimal medium for inducing safflower leaves into healing tissue was MS+6-BA 3 mg/L+NAA 0.5 mg/L,and the healing rate reached 91.3%. After 20 d of culture, the best medium for inducing leaf healing tissue to differentiate into adventitious shoots was MS+KT 3.0 mg/L+IAA 0.05 mg/L. After 30 d of culture, the best medium for secondary proliferation was MS+KT 3.0 mg/L+IAA 0.1 mg/L, and the coefficient of proliferation was up to 111.7. The best medium for in-bottle rooting was MS+NAA 0.1 mg/L+ IBA 0.2 mg/L.The best medium for in-bottle rooting was MS+NAA 0.1 mg/L+ IBA 0.2 mg/L. IBA 0.2 mg/L, and the rooting rate reached 90.9%. 【Conclusion】In this study, the most suitable hormone ratio for the growth of safflower explants is screened out, which has provided technical support for the establishment of safflower tissue culture and 60 d propagation system, the acquisition of intact plants as well as the screening of highquality germplasm resources of safflower.更多还原