【摘要】 目的 对比聚乙二醇沉淀法与磁珠法在城市污水新型冠状病毒（SARS-Co V-2）检测中的效能差异，为新型冠状病毒预警监测和科学防控提供理论依据。方法 通过新型冠状病毒标准物质梯度稀释得到标准曲线，计算聚乙二醇沉淀法和磁珠法的回收率，并采用χ²检验、Wilcoxon秩和检验来比较2种方法对污水中新冠病毒的检出率与检出浓度。结果 在加标试验中，聚乙二醇沉淀法和磁珠法对Orf1a/b基因回收率分别为3.08%和18.74%,N基因的回收率分别为3.46%和21.52%。采用聚乙二醇沉淀法、磁珠法对414份污水样本分别进行检测，Orf1a/b基因的检出率分别为37.92%和43.24%,N基因的检出率分别为63.29%和67.39%，差异均无统计学意义（Orf1a/b基因：χ²=1.025,P=0.311;N基因：χ²=0.323,P=0.570）;Orf1a/b基因检出浓度M(P₂₅～P₇₅)分别为0.70（0.38～1.41）copies/m L和3.01（1.10～6.31）copies/m L,N基因检出浓度M(P₂₅～P₇₅)分别为4.43（2.18～12.54）copies/m L和18.11（6.77～50.41）copies/m L，差异均有统计学意义（Orf1a/b基因：Z=-12.977,P<0.001;N基因：Z=-14.821,P<0.001）。结论 2种富集方法在城市污水中新冠病毒的检出率无明显差异，但磁珠法的回收率更高，2种富集方法均可为病毒监测提供数据支持。更多还原

【Abstract】 Objective To compare the efficacy of the polyethylene glycol precipitation method and the magnetic bead method in the detection of novel coronavirus (SARS-Co V-2) in municipal sewage,and to provide a theoretical basis for the early monitoring and scientific prevention and control of SARS-Co V-2.Methods The standard curve was obtained by gradient dilution of the SARS-CoV-2 standards.The recovery rate of the polyethylene glycol precipitation method and the magnetic bead method were calculated.Both the χ~2 test and the Wilcoxon nonparametric rank-sum test were applied to calculate the detection rate and concentration of the SARA-CoV-2.Results In the spiking experiments,the recoveries of Orf1a/b genes were 3.08% and 18.74% by polyethylene glycol precipitation and magnetic bead method,while those of the N gene were 3.46% and 21.52% ,respectively.A total of 414 sewage samples were detected using polyethylene glycol precipitation and magnetic bead method respectively.The detection rates of Orf1a/b genes were 37.92% and 43.24% ,respectively,and those of N genes were 63.29% and 67.39% ,respectively,which showed no significant differences (Orf1a/b gene: χ~2 =1.025,P=0.311;N gene: χ~2 =0.323,P=0.570).The detected concentrations M (P25～P75) of the Orf1a/b genes were 0.70 (0.38～1.41)copies/m L and 3.01 (1.10～6.31) copies/m L,respectively,while those of the N gene were 4.43 (2.18-12.54) copies/m L and18.11 (6.77～50.41) copies/m L,respectively.The differences were all statistically significant (for the Orf1a/b gene:Z=-12.977,P<0.001;for the N gene:Z=-14.821,P<0.001).Conclusions There was no significant difference in the detection rate of SARA-CoV-2 in municipal sewage between the two enrichment methods,but the recovery rate of the magnetic bead method was higher.Both the enrichment methods can provide data support for virus monitoring.更多还原