【摘要】 目的 探讨miR-181a与ATG5在乳腺癌患者中的表达情况，深入研究其对MCF-7乳腺癌细胞系自噬的调控作用，以揭示其在乳腺癌发展中的临床意义和潜在机制。方法 选取2021年6月—2022年9月本院收治的120例乳腺癌患者作为疾病组，以及55名健康体检者作为正常对照组。使用qPCR检测血清和组织中miR-181a和ATG5的表达。CCK-8检测了MCF-7细胞增殖能力的变化，Western blot检测MCF-7细胞自噬和凋亡相关蛋白的表达。流式细胞术检测MCF-7细胞各组凋亡率。结果 乳腺癌患者血清和组织中miR-181a的表达显著增加，而ATG5表达显著降低(P<0.01)。miR-181a的表达与乳腺癌的淋巴结转移、TNM分期和肿瘤分化程度密切相关(P<0.01)。抑制miR-181a表达可以显著降低MCF-7细胞的增殖能力，促进自噬相关蛋白ATG5、LC3B和Beclin-1的表达，促进凋亡BAX/BCL-2蛋白比值的表达。结论 miR-181a的上调与乳腺癌的临床特征相关，可能作为潜在的标志物用于病情评估。此外miR-181a可能通过调节自噬和凋亡来影响乳腺癌细胞的增殖和存活，有望为乳腺癌的诊断和治疗提供新的研究方向和临床应用前景。更多还原

【Abstract】 Objective To investigate the expression of miR-181a and ATG5 in breast cancer patients, and further study their regulatory effects on autophagy of MCF-7 breast cancer cell line, in order to reveal their clinical significance and potential mechanism in the development of breast cancer. Methods In this study, 120 patients with breast cancer admitted from June 2021 to September 2022 were selected as disease group and 55 healthy subjects were selected as normal control group. The expression of miR-181a and ATG5 in serum and tissues was detected by qPCR. CCK-8 was used to detect the changes in the proliferation ability of MCF-7 cells, and Western blot was used to detect the expression of autophagy and apoptosis-related proteins in MCF-7 cells. The apoptosis rate of MCF-7 cells in each group was detected by flow cytometry. Results The expression of miR-181a in serum and tissues of breast cancer patients was significantly increased, while the expression of ATG5 was significantly decreased(P<0.01). The expression of miR-181a was closely related to lymph node metastasis, TNM stage and tumor differentiation(P<0.01). Inhibition of miR-181a expression could significantly reduce the proliferation capacity of MCF-7 cells, promote the expression of autophagy related proteins ATG5, LC3B and Beclin-1, and promote the expression of apoptotic BAX/BCL-2 protein ratio. Conclusion The up-regulation of miR-181a is associated with the clinical characteristics of breast cancer and may be used as a potential marker for disease assessment. In addition, miR-181a may affect the proliferation and survival of breast cancer cells by regulating autophagy and apoptosis. It is expected to provide a new research direction and clinical application prospect for the diagnosis and treatment of breast cancer.更多还原